SPIM流体：基于光片的开源高通量成像平台。
SPIM-fluid: open source light-sheet based platform for high-throughput imaging.
作者信息
Gualda Emilio J, Pereira Hugo, Vale Tiago, Estrada Marta Falcão, Brito Catarina, Moreno Nuno
机构信息
Advance Imaging Lab, Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6,Oeiras 2780-156, Portugal.
Advance Imaging Lab, Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6,Oeiras 2780-156, Portugal ; iBET - Instituto de Biologia Experimental e Tecnológica, Oeiras 2781-901, Portugal ; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras 2780-157, Portugal.
出版信息
Biomed Opt Express. 2015 Oct 19;6(11):4447-56. doi: 10.1364/BOE.6.004447. eCollection 2015 Nov 1.
Abstract
Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening.
摘要
光片荧光显微镜最近已成为获取全生物体/胚胎高质量3D图像的首选技术，具有低光损伤和快速采集速率。在此，我们展示了一种基于Arduino和MicroManager的开源统一实现方案，它能够操作光片显微镜，对三维细胞培养物和斑马鱼等模式生物进行自动化3D高通量成像，以用于大规模药物筛选。
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