Liu Bingqian, Tian Mengmeng, Guo Qin, Ma Lei, Zhou Dan, Shen Bo, Sun Yan, Zhu Changliang
Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, Jiangsu 210029, China (
J Med Entomol. 2016 Sep 1;53(5):1205-1210. doi: 10.1093/jme/tjw083.
MicroRNAs (miRNAs) play notable role in regulation of gene expression at the posttranscription level, and have been involved in many biological processes, including insecticide resistance. In this study, we investigated the role of miR-932 in the molecular mechanisms of pyrethroid resistance in Culex pipiens pallens (L.). Overexpression of miR-932 in the DS-strain made the mosquitoes more resistant to deltamethrin, while inhibiting the expression of miR-932 in the DR-strain made the mosquitoes more sensitive to deltamethrin. Further, we also identified CpCPR5 as a target gene of miR-932. Sustained overexpression of miR-932 resulted in repression of CpCPR5, and that knockdown of miR-932 increased CpCPR5 expression. In addition, knockdown of CpCPR5 decreased the sensitivity of mosquitoes to deltamethrin in the DS-strain. In conclusion, our study finds a molecular link between miR-932 and CpCPR5 and provides a novel insight into the mechanism of insecticide resistance.
微小RNA（miRNA）在转录后水平的基因表达调控中发挥着显著作用，并参与了包括抗杀虫剂在内的许多生物学过程。在本研究中，我们调查了miR-932在淡色库蚊对拟除虫菊酯抗性分子机制中的作用。在DS品系中过表达miR-932使蚊子对溴氰菊酯更具抗性，而在DR品系中抑制miR-932的表达则使蚊子对溴氰菊酯更敏感。此外，我们还鉴定出CpCPR5是miR-932的一个靶基因。持续过表达miR-932导致CpCPR5受到抑制，而敲低miR-932则增加了CpCPR5的表达。另外，敲低DS品系中蚊子的CpCPR5会降低其对溴氰菊酯的敏感性。总之，我们的研究发现了miR-932与CpCPR5之间的分子联系，并为杀虫剂抗性机制提供了新的见解。
