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木薯 CC 型谷氧还蛋白 MeGRXC3 通过调控其核活性影响甘露醇诱导的渗透胁迫敏感性的作用。

Role of cassava CC-type glutaredoxin MeGRXC3 in regulating sensitivity to mannitol-induced osmotic stress dependent on its nuclear activity.

机构信息

Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.

Key Laboratory of Biology and Genetic Resources of Torpical Crops, Ministry of Agriculture, Haikou, 571101, China.

出版信息

BMC Plant Biol. 2022 Jan 20;22(1):41. doi: 10.1186/s12870-022-03433-y.

DOI:10.1186/s12870-022-03433-y
PMID:35057736
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8772167/
Abstract

BACKGROUND

We previously identified six drought-inducible CC-type glutaredoxins in cassava cultivars, however, less is known about their potential role in the molecular mechanism by which cassava adapted to abiotic stress.

RESULTS

Herein, we investigate one of cassava drought-responsive CC-type glutaredoxins, namely MeGRXC3, that involved in regulation of mannitol-induced inhibition on seed germination and seedling growth in transgenic Arabidopsis. MeGRXC3 overexpression up-regulates several stress-related transcription factor genes, such as PDF1.2, ERF6, ORA59, DREB2A, WRKY40, and WRKY53 in Arabidopsis. Protein interaction assays show that MeGRXC3 interacts with Arabidopsis TGA2 and TGA5 in the nucleus. Eliminated nuclear localization of MeGRXC3 failed to result mannitol-induced inhibition of seed germination and seedling growth in transgenic Arabidopsis. Mutation analysis of MeGRXC3 indicates the importance of conserved motifs for its transactivation activity in yeast. Additionally, these motifs are also indispensable for its functionality in regulating mannitol-induced inhibition of seed germination and enhancement of the stress-related transcription factors in transgenic Arabidopsis.

CONCLUSIONS

MeGRXC3 overexpression confers mannitol sensitivity in transgenic Arabidopsis possibly through interaction with TGA2/5 in the nucleus, and nuclear activity of MeGRXC3 is required for its function.

摘要

背景

我们之前在木薯品种中鉴定了六个干旱诱导的 CC 型谷氧还蛋白，但对它们在木薯适应非生物胁迫的分子机制中的潜在作用知之甚少。

结果

在此，我们研究了木薯干旱响应的 CC 型谷氧还蛋白之一，即 MeGRXC3，它参与调控甘露醇诱导的拟南芥种子萌发和幼苗生长抑制。MeGRXC3 的过表达上调了几个与胁迫相关的转录因子基因，如 PDF1.2、ERF6、ORA59、DREB2A、WRKY40 和 WRKY53 在拟南芥中。蛋白相互作用分析表明，MeGRXC3 在核内与拟南芥 TGA2 和 TGA5 相互作用。消除 MeGRXC3 的核定位未能导致转基因拟南芥中甘露醇诱导的种子萌发和幼苗生长抑制。MeGRXC3 的突变分析表明，保守基序对于其在酵母中的转录激活活性很重要。此外，这些基序对于其在调控甘露醇诱导的种子萌发抑制和增强转基因拟南芥中与胁迫相关的转录因子方面的功能也是必不可少的。

结论

MeGRXC3 的过表达使转基因拟南芥对甘露醇敏感，可能是通过与核内的 TGA2/5 相互作用，而 MeGRXC3 的核活性是其功能所必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/95053d5a95af/12870_2022_3433_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/9f1682fd92c0/12870_2022_3433_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/cd18374f7a3a/12870_2022_3433_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/61d1829fbd8b/12870_2022_3433_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/54eac848acd7/12870_2022_3433_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/95053d5a95af/12870_2022_3433_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/9f1682fd92c0/12870_2022_3433_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/1308378816f0/12870_2022_3433_Fig2_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/cd18374f7a3a/12870_2022_3433_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/61d1829fbd8b/12870_2022_3433_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/54eac848acd7/12870_2022_3433_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b92/8772167/95053d5a95af/12870_2022_3433_Fig7_HTML.jpg

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