Francisco Marta, Joseph Bindu, Caligagan Hart, Li Baohua, Corwin Jason A, Lin Catherine, Kerwin Rachel, Burow Meike, Kliebenstein Daniel J
Department of Plant Sciences, University of CaliforniaDavis, CA, USA; Group of Genetics, Breeding and Biochemistry of Brassicas, Department of Plant Genetics, Misión Biológica de Galicia, Spanish Council for Scientific ResearchPontevedra, Spain.
Department of Plant Sciences, University of California Davis, CA, USA.
Front Plant Sci. 2016 Jun 1;7:774. doi: 10.3389/fpls.2016.00774. eCollection 2016.
Glucosinolates (GSLs) play an important role in plants as direct mediators of biotic and abiotic stress responses. Recent work is beginning to show that the GSLs can also inducing complex defense and growth networks. However, the physiological significance of these GSL-induced responses and the molecular mechanisms by which GSLs are sensed and/or modulate these responses are not understood. To identify these potential mechanisms within the plant and how they may relate to the endogenous GSLs, we tested the regulatory effect of exogenous allyl GSL application on growth and defense metabolism across sample of Arabidopsis thaliana accessions. We found that application of exogenous allyl GSL had the ability to initiate changes in plant biomass and accumulation of defense metabolites that genetically varied across accessions. This growth effect was related to the allyl GSL side-chain structure. Utilizing this natural variation and mutants in genes within the GSL pathway we could show that the link between allyl GSL and altered growth responses are dependent upon the function of known genes controlling the aliphatic GSL pathway.
硫代葡萄糖苷（GSLs）在植物中作为生物和非生物胁迫反应的直接介质发挥着重要作用。最近的研究开始表明，GSLs还能诱导复杂的防御和生长网络。然而，这些GSL诱导反应的生理意义以及GSLs感知和/或调节这些反应的分子机制尚不清楚。为了确定植物体内的这些潜在机制以及它们与内源性GSLs的关系，我们测试了外源烯丙基GSL应用对拟南芥不同生态型样本生长和防御代谢的调节作用。我们发现，外源烯丙基GSL的应用能够引发植物生物量的变化以及防御代谢物的积累，这些变化在不同生态型中存在遗传差异。这种生长效应与烯丙基GSL的侧链结构有关。利用这种自然变异和GSL途径内基因的突变体，我们可以表明烯丙基GSL与生长反应改变之间的联系取决于控制脂肪族GSL途径的已知基因的功能。
