Xie Qiao, Jia Lie-Ni, Xu Hong-Yu, Hu Xiang-Gang, Wang Wei, Jia Jun
State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, China; No. 422 Hospital of PLA, Zhanjiang, Guangdong 524005, China.
State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Stem Cells Int. 2016;2016:5385137. doi: 10.1155/2016/5385137. Epub 2016 May 26.
Bone tissue engineering is the most promising technology for enhancing bone regeneration. Scaffolds loaded with osteogenic factors improve the therapeutic effect. In this study, the bioactive PEI (polyethylenimine)/pBMP2- (bone morphogenetic protein-2 plasmid-) PLGA (poly(D, L-lactic-co-glycolic acid)) core-shell scaffolds were prepared using coaxial electrospinning for a controlled gene delivery to hPDLSCs (human periodontal ligament stem cells). The pBMP2 was encapsulated in the PEI phase as a core and PLGA was employed to control pBMP2 release as a shell. First, the scaffold characterization and mechanical properties were evaluated. Then the gene release behavior was analyzed. Our results showed that pBMP2 was released at high levels in the first few days, with a continuous release behavior in the next 28 days. At the same time, PEI/pBMP2 showed high transfection efficiency. Moreover, the core-shell electrospun scaffold showed BMP2 expression for a much longer time (more than 28 days) compared with the single axial electrospun scaffold, as evaluated by qRT-PCR and western blot after culturing with hPDLSCs. These results suggested that the core-shell PEI/pBMP2-PLGA scaffold fabricated by coaxial electrospinning had a good gene release behavior and showed a prolonged expression time with a high transfection efficiency.
骨组织工程是促进骨再生最具前景的技术。负载成骨因子的支架可提高治疗效果。在本研究中，采用同轴静电纺丝制备了生物活性聚乙二胺（PEI）/骨形态发生蛋白-2质粒（pBMP2）-聚（D，L-乳酸-乙醇酸共聚物）（PLGA）核壳支架，用于向人牙周膜干细胞（hPDLSCs）进行可控基因递送。pBMP2被包裹在PEI相作为核心，PLGA作为外壳用于控制pBMP2的释放。首先，对支架的特性和力学性能进行了评估。然后分析了基因释放行为。我们的结果表明，pBMP2在最初几天高水平释放，随后28天持续释放。同时，PEI/pBMP2显示出高转染效率。此外，与单轴静电纺丝支架相比，在与hPDLSCs共培养后通过qRT-PCR和蛋白质印迹评估发现，核壳静电纺丝支架显示BMP2表达的时间长得多（超过28天）。这些结果表明，通过同轴静电纺丝制备的核壳PEI/pBMP2-PLGA支架具有良好的基因释放行为，显示出延长的表达时间和高转染效率。
