Morshedi Dina, Nasouti Mahour
National Institute of Genetic Engineering and Biotechnology, Department of Industrial & Environmental Biotechnology, Shahrak-e Pajoohesh, km 15, Tehran-Karaj Highway, P.O. Box 14965/161, Tehran, Iran.
Parkinsons Dis. 2016;2016:6219249. doi: 10.1155/2016/6219249. Epub 2016 May 24.
α-Synuclein (α-Syn) fibrillation links with Parkinson's disease (PD) and several related syndromes. It is believed that exposure to the factors which promote fibrillation may induce and progress such neurodegenerative diseases (NDs). Herein, the effects of some wildly used essential oils including Myrtus communis (M. communis) on α-Syn fibrillation were examined. M. communis particularly increased α-Syn fibrillation in a concentration dependent manner. Given that applications of M. communis are very extensive in Asian societies, especially Zoroastrians, this study was extended towards its role on α-Syn fibrillation/cytotoxicity. By using a unilamellar vesicle, it was shown that the aggregated species with tendency to perturb membrane were increased in the presence of M. communis. In this regard, the cytotoxicity of α-Syn on SH-SH5Y cells was also increased significantly. Inappropriately, the effects of fibrillation inhibitors, baicalein and cuminaldehyde, were modulated in the presence of M. communis. However, major components of M. communis did not induce fibrillation and also the effect of M. communis was limited on other fibrinogenic proteins. Assuming that essential oils have the ability to pass through the blood brain barrier (BBB) along with the popular attention on aromatherapy for the incurable ND, these findings suggest an implementation of fibrillation tests for essential oils.
α-突触核蛋白（α-Syn）纤维化与帕金森病（PD）及几种相关综合征有关。据信，接触促进纤维化的因素可能会诱发并促使这些神经退行性疾病（NDs）发展。在此，研究了包括香桃木（Myrtus communis）在内的一些广泛使用的精油对α-Syn纤维化的影响。香桃木尤其以浓度依赖的方式增加α-Syn纤维化。鉴于香桃木在亚洲社会，特别是拜火教徒中的应用非常广泛，本研究扩展到其对α-Syn纤维化/细胞毒性的作用。通过使用单层囊泡，结果表明在香桃木存在的情况下，倾向于扰乱膜的聚集物增加。在这方面，α-Syn对SH-SH5Y细胞的细胞毒性也显著增加。不合适的是，在香桃木存在的情况下，纤维化抑制剂黄芩素和枯茗醛的作用受到调节。然而，香桃木的主要成分不会诱导纤维化，并且香桃木对其他纤维蛋白原性蛋白的作用也有限。鉴于精油有能力穿过血脑屏障（BBB），以及人们对不可治愈的神经退行性疾病进行芳香疗法的普遍关注，这些发现表明应对精油进行纤维化测试。
