Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA.
Department of Otolaryngology/Head and Neck Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA and The Curriculum of Neurobiology, University of North Carolina, Chapel Hill, NC, USA and Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC, USA.
Analyst. 2016 Aug 2;141(16):4902-11. doi: 10.1039/c6an00933f.
Multi-modal recording describes the simultaneous collection of information across distinct domains. Compared to isolated measurements, such studies can more easily determine relationships between varieties of phenomena. This is useful for neurochemical investigations which examine cellular activity in response to changes in the local chemical environment. In this study, we demonstrate a method to perform simultaneous patch clamp measurements with fast-scan cyclic voltammetry (FSCV) using optically isolated instrumentation. A model circuit simulating concurrent measurements was used to predict the electrical interference between instruments. No significant impact was anticipated between methods, and predictions were largely confirmed experimentally. One exception was due to capacitive coupling of the FSCV potential waveform into the patch clamp amplifier. However, capacitive transients measured in whole-cell current clamp recordings were well below the level of biological signals, which allowed the activity of cells to be easily determined. Next, the activity of medium spiny neurons (MSNs) was examined in the presence of an FSCV electrode to determine how the exogenous potential impacted nearby cells. The activities of both resting and active MSNs were unaffected by the FSCV waveform. Additionally, application of an iontophoretic current, used to locally deliver drugs and other neurochemicals, did not affect neighboring cells. Finally, MSN activity was monitored during iontophoretic delivery of glutamate, an excitatory neurotransmitter. Membrane depolarization and cell firing were observed concurrently with chemical changes around the cell resulting from delivery. In all, we show how combined electrophysiological and electrochemical measurements can relate information between domains and increase the power of neurochemical investigations.
多模态记录描述了跨不同领域的信息同时采集。与孤立的测量相比，此类研究更容易确定各种现象之间的关系。这对于神经化学研究很有用，神经化学研究检查细胞活动对局部化学环境变化的反应。在这项研究中，我们展示了一种使用光学隔离仪器同时进行膜片钳测量和快速扫描循环伏安法 (FSCV) 的方法。使用模拟并发测量的模型电路来预测仪器之间的电干扰。预计两种方法之间没有重大影响，并且预测在很大程度上得到了实验证实。一个例外是由于 FSCV 电位波形电容耦合到膜片钳放大器中。然而，在全细胞电流钳记录中测量的电容瞬变远低于生物信号的水平，这允许轻松确定细胞的活动。接下来，检查了存在 FSCV 电极时中脑神经元 (MSN) 的活动，以确定外源性电位如何影响附近的细胞。FSCV 波形对静息和活动 MSN 的活动均无影响。此外，局部输送药物和其他神经化学物质的离子电渗流应用不会影响邻近细胞。最后，在离子电渗递谷氨酸（一种兴奋性神经递质）期间监测 MSN 活动。观察到膜去极化和细胞放电与细胞周围的化学变化同时发生，这些化学变化是由于递药引起的。总之，我们展示了如何将电生理和电化学测量相结合，以在不同领域之间关联信息并增强神经化学研究的能力。
