Krutty John D, Schmitt Samantha K, Gopalan Padma, Murphy William L
Department of Biomedical Engineering, University of Wisconsin-Madison, 53706, USA.
Department of Materials Science and Engineering, University of Wisconsin-Madison, 53706, USA.
Curr Opin Biotechnol. 2016 Aug;40:164-169. doi: 10.1016/j.copbio.2016.05.006. Epub 2016 Jun 14.
The promise of growing tissues to replace or improve the function of failing ones, a practice often referred to as regenerative medicine, has been driven in recent years by the development of stem cells and cell lines. Stem cells are typically cultured outside the body to increase cell number or differentiate the cells into mature cell types. In order to maximize the regenerative potential of these cells, there is a need to understand cell-material interactions that direct cell behavior and cell-material dynamics. Most synthetic surfaces used for growth and differentiation of cells in the lab are impractical and cost prohibitive in clinical labs. This review focuses on the modification of low cost polymer substrates that are already widely used for cell culture so that they may be used to control and understand cell-material interactions. In addition, we discuss the ability of cells to exert dynamic control over the microenvironment leading to a more complex, less controlled surface.
通过生长组织来替代或改善功能衰竭组织的功能，这种做法通常被称为再生医学，近年来受到干细胞和细胞系发展的推动。干细胞通常在体外培养，以增加细胞数量或将细胞分化为成熟的细胞类型。为了最大限度地发挥这些细胞的再生潜力，有必要了解指导细胞行为和细胞-材料动态的细胞-材料相互作用。实验室中用于细胞生长和分化的大多数合成表面在临床实验室中不实用且成本高昂。本综述重点关注对已广泛用于细胞培养的低成本聚合物基质的改性，以便它们可用于控制和理解细胞-材料相互作用。此外，我们还讨论了细胞对微环境施加动态控制的能力，从而导致表面更加复杂、难以控制。
