Palya Vilmos, Zolnai Anna, Benyeda Zsófia, Kovács Edit, Kardi Veronika, Mató Tamás
CEVA-Phylaxia Veterinary Biologicals Co. Ltd, Budapest, Hungary.
Avian Pathol. 2009 Apr;38(2):175-80. doi: 10.1080/03079450902737839.
From the early 1970s to the present, numerous cases of short beak and dwarfism syndrome (SBDS) have been reported in mule ducks from France. The animals showed strong growth retardation with smaller beak and tarsus. It was suggested that the syndrome was caused by goose parvovirus on the basis of serological investigation, but the causative agent has not been isolated and the disease has not so far been reproduced by experimental infection. The aim of the present study was to characterize the virus strains isolated from field cases of SBDS, and to reproduce the disease experimentally. Phylogenetic analysis proved that the parvovirus isolates obtained from SBDS of mule duck belonged to a distinct lineage of goose parvovirus-related group of waterfowl parvoviruses. The authors carried out experimental infections of 1-day-old, 2-week-old and 3-week-old mule ducks by the oral route with three different parvovirus strains: strain D17/99 of goose parvovirus from Derzsy's disease, strain FM of Muscovy duck parvovirus from the parvovirus disease of Muscovy ducks, and strain D176/02 isolated from SBDS of mule duck. The symptoms of SBDS of the mule duck could only be reproduced with the mule duck isolate (strain D176/02) following 1-day-old inoculation. Infection with a genetically different strain of goose parvovirus isolated from classical Derzsy's disease (D17/99) or with the Muscovy duck parvovirus strain (FM) did not cause any clinical symptoms or pathological lesions in mule ducks.
从20世纪70年代初至今，法国的骡鸭中已报告了大量短喙和侏儒综合征（SBDS）病例。这些动物表现出严重的生长迟缓，喙和跗骨较小。血清学调查表明该综合征是由鹅细小病毒引起的，但病原体尚未分离出来，且迄今为止尚未通过实验感染再现该疾病。本研究的目的是对从SBDS野外病例中分离出的病毒株进行特征分析，并通过实验再现该疾病。系统发育分析证明，从骡鸭SBDS中获得的细小病毒分离株属于水禽细小病毒中与鹅细小病毒相关的一个独特谱系。作者用三种不同的细小病毒株经口感染1日龄、2周龄和3周龄的骡鸭：来自Derzsy病的鹅细小病毒D17/99株、来自番鸭细小病毒病的番鸭细小病毒FM株以及从骡鸭SBDS中分离出的D176/02株。只有在接种1日龄骡鸭后，用骡鸭分离株（D176/02株）才能再现骡鸭SBDS的症状。用从经典Derzsy病中分离出的基因不同的鹅细小病毒株（D17/99）或番鸭细小病毒株（FM）感染骡鸭，未引起任何临床症状或病理损伤。
