Cousins S W, Altman N H, Atherton S S
Department of Microbiology and Immunology, University of Miami School of Medicine, Florida 33101.
Exp Eye Res. 1989 Jun;48(6):745-60. doi: 10.1016/0014-4835(89)90061-4.
Injection of the KOS strain of herpes simplex virus type 1 into the anterior chamber of one eye of a BALB/c mouse produces bilateral retinal inflammation which progresses in the contralateral eye to total retinal necrosis by day 10 post-inoculation. Although both immune and direct viral cytopathic effects have been proposed as causes of the initial infiltration, the precise mechanism(s) of the contralateral retinal necrosis remains uncertain. In this study, histopathological examination of the uninoculated eyes of HSV-1 injected BALB/c mice revealed the presence of vitreous traction against the internal limiting membrane of the retina which was associated with separation of the nerve fiber layer from the underlying ganglion cell layer. This separation resulted in avulsion of Mueller cell footplates and retinal vessels, hemorrhage into the schisis cavity and disruption of the underlying retinal architecture. The schisis began focally on day 8 or 9 post-inoculation, and the entire retina of most animals was involved by day 10 or 11. These results suggest that retinal schisis involving separation of the nerve fiber layer from the ganglion cell layer contributes to necrosis of the retina in the uninoculated contralateral eye.
将1型单纯疱疹病毒的KOS株注射到BALB/c小鼠的一只眼睛前房，会引发双侧视网膜炎症，在接种后第10天，对侧眼睛的视网膜炎症会发展为完全坏死。虽然免疫和直接病毒细胞病变效应都被认为是初始浸润的原因，但对侧视网膜坏死的确切机制仍不确定。在本研究中，对注射HSV-1的BALB/c小鼠未接种的眼睛进行组织病理学检查，发现玻璃体对视网膜内界膜有牵引作用，这与神经纤维层与下方神经节细胞层分离有关。这种分离导致米勒细胞脚板和视网膜血管撕脱，血液进入劈裂腔，并破坏下方的视网膜结构。劈裂在接种后第8或9天开始局部出现，到第10或11天，大多数动物的整个视网膜都受到影响。这些结果表明，涉及神经纤维层与神经节细胞层分离的视网膜劈裂是对侧未接种眼睛视网膜坏死的原因。
