Azizi Susan, Mohamad Rosfarizan, Bahadoran Azadeh, Bayat Saadi, Rahim Raha Abdul, Ariff Arbakariya, Saad Wan Zuhainis
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia.
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia; Institute of Tropical Forestry and Forest Products (INTROP), Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia.
J Photochem Photobiol B. 2016 Aug;161:441-9. doi: 10.1016/j.jphotobiol.2016.06.007. Epub 2016 Jun 7.
The use of nontoxic biological compounds in the synthesis of nanomaterials is an economic and eco-friendly approach. The present work was undertaken to develop zinc oxide nanoparticles (ZnO-NPs) by a green method using simple precursor from the solution consisting of zinc acetate and the flower extract of Anchusa italica (A. italica). Effect of annealing temperature on structural and antimicrobial properties was investigated. The crystalline structure of ZnO-NPs was shown using X-ray diffraction (XRD) analysis. Transmission electron microscopy (TEM) results showed that ZnO-NPs are hexagonal in shapes with mean particle size of ~8 and ~14nm at 100°C and 200°C annealing temperatures respectively. The optical band gap was increased from 3.27eV to 3.30eV with the decreasing of the particle size. The antimicrobial activity of ZnO-NPs towards Gram positive (Bacillus megaterium and Stapphylococcus aureus) and Gram negative (Escherichia coli and Salmonella typhimurium) pathogens decreased with the increasing of the heat treating temperature. In vitro cytotoxicity studies on Vero cells, a dose dependent toxicity with non-toxic effect of concentration below 142μg/mL was shown. The results indicated that A. italica is an appropriate reaction media to prepare ZnO-NPs for cosmetic and bio-medical productions.
在纳米材料合成中使用无毒生物化合物是一种经济且环保的方法。本研究旨在通过绿色方法，使用由醋酸锌和意大利牛舌草（Anchusa italica）花提取物组成的简单前驱体溶液来制备氧化锌纳米颗粒（ZnO-NPs）。研究了退火温度对其结构和抗菌性能的影响。通过X射线衍射（XRD）分析展示了ZnO-NPs的晶体结构。透射电子显微镜（TEM）结果表明，在100°C和200°C退火温度下，ZnO-NPs分别呈六边形，平均粒径约为8nm和14nm。随着粒径减小，光学带隙从3.27eV增加到3.30eV。ZnO-NPs对革兰氏阳性菌（巨大芽孢杆菌和金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌和鼠伤寒沙门氏菌）病原体的抗菌活性随着热处理温度的升高而降低。对Vero细胞的体外细胞毒性研究表明，存在剂量依赖性毒性，浓度低于142μg/mL时无毒性作用。结果表明，意大利牛舌草是制备用于化妆品和生物医学产品的ZnO-NPs的合适反应介质。
