Azizi Susan, Mohamad Rosfarizan, Mahdavi Shahri Mahnaz
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia.
Laboratory of Biopolymer and Derivatives, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia.
Molecules. 2017 Feb 16;22(2):301. doi: 10.3390/molecules22020301.
In this paper, a green microwave-assisted combustion approach to synthesize ZnO-NPs using zinc nitrate and (L.) Schrad (fruit, seed and pulp) extracts as bio-fuels is reported. The structure, optical, and colloidal properties of the synthesized ZnO-NP samples were studied. Results illustrate that the morphology and particle size of the ZnO samples are different and depend on the bio-fuel. The XRD results revealed that hexagonal wurtzite ZnO-NPs with mean particle size of 27-85 nm were produced by different bio-fuels. The optical band gap was increased from 3.25 to 3.40 eV with the decreasing of particle size. FTIR results showed some differences in the surface structures of the as-synthesized ZnO-NP samples. This led to differences in the zeta potential, hydrodynamic size, and more significantly, antioxidant activity through scavenging of 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) free radicals. In in vitro cytotoxicity studies on 3T3 cells, a dose dependent toxicity with non-toxic effect of concentration below 0.26 mg/mL was shown for ZnO-NP samples. Furthermore, the as-synthesized ZnO-NPs inhibited the growth of medically significant pathogenic gram-positive ( and Methicillin-resistant ) and gram-negative ( and ) bacteria. This study provides a simple, green and efficient approach to produce ZnO nanoparticles for various applications.
本文报道了一种绿色微波辅助燃烧法，以硝酸锌和（L.）Schrad（果实、种子和果肉）提取物作为生物燃料来合成ZnO纳米颗粒。研究了合成的ZnO-NP样品的结构、光学和胶体性质。结果表明，ZnO样品的形态和粒径不同，且取决于生物燃料。XRD结果显示，不同生物燃料制备出了平均粒径为27-85nm的六方纤锌矿型ZnO纳米颗粒。随着粒径减小，光学带隙从3.25eV增加到3.40eV。FTIR结果表明，合成的ZnO-NP样品的表面结构存在一些差异。这导致了zeta电位、流体动力学尺寸的差异，更显著的是，通过清除1,1-二苯基-2-苦基肼（DPPH）自由基，抗氧化活性也有所不同。在对3T3细胞的体外细胞毒性研究中，ZnO-NP样品显示出剂量依赖性毒性，浓度低于0.26mg/mL时无毒性作用。此外，合成的ZnO纳米颗粒抑制了具有医学意义的致病性革兰氏阳性菌（和耐甲氧西林）和革兰氏阴性菌（和）的生长。本研究提供了一种简单、绿色且高效的方法来制备用于各种应用的ZnO纳米颗粒。
