Jinno K, Moriwaki S, Govindarajan S, Okada Y, Tsuji T
Department of Internal Medicine and Clinical Research, National Hospital Shikoku Cancer Center, Matsuyama, Japan.
J Hepatol. 1989 May;8(3):330-7. doi: 10.1016/0168-8278(89)90031-7.
The expression of the carbohydrate antigens related to type 1 chain N-acetyllactosamine (1NAcLc) in the proliferated bile ductules was immunohistochemically examined in liver tissues of 10 cases of chronic hepatitis (CH), 9 of liver cirrhosis (LC) and 8 of alcoholic hepatitis. The ductular expression of the examined blood group antigens was essentially the same among these pathological conditions. The backbone structure, i.e., 1NAcLc (Gal beta 1----3GlcNAc beta 1----3Gal beta 1----3R), was not detected in the proliferated ductules as in the normal bile ductules. Its fucosylated structures (Le(a), Le(b) and type 1 chain H) were more strongly expressed in the proliferated ductules than in the normal ductules. Sialyl-Le(a) which was not found in the normal ductules was detected weakly but definitely in the proliferated ductules. Besides proliferated ductules, single or small numbers of epithelial cells expressing the 1NAcLc-related antigens were found intralobularly. This suggests possible migration of biliary epithelial cells into the hepatic lobules. In conclusion, within the proliferated bile ductules (a) synthesis of the 1NAcLc-related antigens is increased compared to the normal ductules, (b) the backbone structure is completely sialylated or fucosylated as in the normal ductules and (c) alpha 1----4fucosylation of sialyl-1NAcLc, i.e., sialyl-Lea, formation occurs despite its absence in the normal ductules.
对10例慢性肝炎（CH）、9例肝硬化（LC）和8例酒精性肝炎患者的肝组织进行免疫组织化学检查，观察增殖性胆小管中与1型链N-乙酰乳糖胺（1NAcLc）相关的碳水化合物抗原的表达情况。在这些病理状态下，所检测血型抗原的胆小管表达基本相同。在增殖性胆小管中未检测到正常胆小管中存在的骨架结构，即1NAcLc（Galβ1----3GlcNAcβ1----3Galβ1----3R）。其岩藻糖基化结构（Le(a)、Le(b)和1型链H）在增殖性胆小管中的表达比正常胆小管更强。正常胆小管中未发现的唾液酸化-Le(a)在增殖性胆小管中虽弱但可明确检测到。除了增殖性胆小管外，在小叶内还发现单个或少量表达1NAcLc相关抗原的上皮细胞。这提示胆管上皮细胞可能向肝小叶迁移。总之，在增殖性胆小管内：（a）与正常胆小管相比，1NAcLc相关抗原的合成增加；（b）骨架结构如正常胆小管一样完全被唾液酸化或岩藻糖基化；（c）尽管正常胆小管中不存在唾液酸化-1NAcLc的α1----4岩藻糖基化，即唾液酸化-Lea，但仍会形成。
