Myint Htun, Kishi Hiroyuki, Koike Satoshi, Kobayashi Yasuo
Graduate School of Agriculture, Hokkaido University, Kita, Sapporo, Japan.
Anim Sci J. 2017 Feb;88(2):372-378. doi: 10.1111/asj.12651. Epub 2016 Jun 21.
Chickpea husk was functionally evaluated for antioxidant status, blood parameters, cecal fermentation and microbial profiles in rats. Fifteen male rats (5 weeks of age) were divided into three groups; they were individually housed and fed one of the following diets for 3 weeks: purified diet containing 5% cellulose (Cellulose), an identical diet in which cellulose was replaced by corn starch (Starch) or by chickpea husk (Chick). Rats were sacrificed to obtain blood and cecal digesta samples. Chickpea husk contained high polyphenolic content and significant superoxide dismutase and 2,2-diphenyl-picrylhydrazyl scavenging activities. In a feeding experiment, Chick showed lowered cholesterol levels and improved antioxidant activity represented by reduced thiobarbituric acid reactive substances in blood. Chick showed increased cecal levels of total short chain fatty acids and butyrate, leading to a lower pH. Chick presented with lowered cecal indole and skatole concentrations, as did Cellulose. Cecal bacterial changes were notable in Chick, evidenced by differences in denaturing gradient gel electrophoresis banding patterns. However, representative bacteria quantified by real-time PCR assay did not support this bacterial change. These results indicate that chickpea husk feeding can improve the antioxidative status of rats through its polyphenolic components and modulate the hindgut environment by its fibrous components.
对鹰嘴豆皮在大鼠体内的抗氧化状态、血液参数、盲肠发酵和微生物谱方面进行了功能评估。将15只5周龄的雄性大鼠分为三组；将它们单独饲养，并喂食以下三种日粮之一，持续3周：含5%纤维素的纯化日粮（纤维素组）、纤维素被玉米淀粉替代的相同日粮（淀粉组）或被鹰嘴豆皮替代的相同日粮（鹰嘴豆皮组）。处死大鼠以获取血液和盲肠消化物样本。鹰嘴豆皮含有高含量的多酚以及显著的超氧化物歧化酶和2,2-二苯基-1-苦基肼清除活性。在一项喂养实验中，鹰嘴豆皮组大鼠的胆固醇水平降低，血液中硫代巴比妥酸反应性物质减少，表明抗氧化活性提高。鹰嘴豆皮组大鼠盲肠中总短链脂肪酸和丁酸盐水平升高，导致pH值降低。鹰嘴豆皮组大鼠盲肠中吲哚和粪臭素浓度降低，纤维素组也是如此。鹰嘴豆皮组大鼠盲肠细菌发生了显著变化，变性梯度凝胶电泳条带模式的差异证明了这一点。然而，通过实时PCR测定法定量的代表性细菌并不支持这种细菌变化。这些结果表明，喂食鹰嘴豆皮可通过其多酚成分改善大鼠的抗氧化状态，并通过其纤维成分调节后肠环境。
