Suda T, Omine M, Tsuchiya J
Scand J Haematol. 1978 Feb;20(2):117-28. doi: 10.1111/j.1600-0609.1978.tb02436.x.
DNA synthesis time (Ts) and 3H-thymidine labelling index (TLI) of erythroblasts have been determined in 25 patients with various types of haematologic disorders using in vitro double labelling method. No remarkable differences in both Ts and TLI were noted between haematologically normal subjects and patients with increased effective erythropoiesis (haemolytic anaemias), suggesting that the cell cycle time is not principally altered under the augmented erythropoiesis. In pernicious anaemia, Ts of basophilic erythroblasts was significantly shortened and TLI was elevated above normal. Normalization of erythropoiesis by vitamin B12 was associated with a transient increase of TLI in polychromatic erythroblasts, which was interpreted to reflect prevention of intramedullary premature death of basophilic megaloblasts. Erythroleukaemia showed a markedly prolonged Ts and lowered TLI indicating the presence of cells with prolonged cell cycle time. These findings contrasted to that of pernicious anaemia despite certain morphological as well as functional similarities. In idiopathic sideroblastic anaemia, prolongation of Ts was observed to a similar extent as erythroleukaemia, while TLI remained almost normal. In 2 cases with suspected erythroleukaemia presenting an intermediate clinical picture between erythroleukaemia and sideroblastic anaemia, Ts of basophilic erythroblasts was found to be prolonged along with modestly lowered TLI.
采用体外双标记法测定了25例各种血液系统疾病患者成红细胞的DNA合成时间（Ts）和3H-胸腺嘧啶核苷标记指数（TLI）。血液学正常受试者与有效红细胞生成增加的患者（溶血性贫血）之间，Ts和TLI均无显著差异，这表明在红细胞生成增加的情况下，细胞周期时间并未发生主要改变。在恶性贫血中，嗜碱性成红细胞的Ts显著缩短，TLI高于正常水平。维生素B12使红细胞生成恢复正常与多色成红细胞中TLI的短暂升高有关，这被解释为反映了对嗜碱性巨幼红细胞髓内过早死亡的预防。红白血病表现出Ts明显延长，TLI降低，表明存在细胞周期时间延长的细胞。尽管在某些形态和功能上有相似之处，但这些发现与恶性贫血的情况形成了对比。在特发性铁粒幼细胞贫血中，Ts延长的程度与红白血病相似，而TLI几乎保持正常。在2例疑似红白血病且临床表现介于红白血病和铁粒幼细胞贫血之间的病例中，发现嗜碱性成红细胞的Ts延长，同时TLI略有降低。
