Queisser W, Pepperl U, Kempgens U, Müller U
Acta Haematol. 1975;54(2):65-74. doi: 10.1159/000208054.
In 6 cases initially showing a symptomatology of erythroleukemia, the proliferation of erythroblasts was studied by combined Feulgen cytophotometry and autoradiography after labelling with 3H-TdR in vivo. A proliferation disturbance was observed consisting of an accumulation of diploid and unlabelled cells and a decreased proportion of cells in S. This defect occurred within the basophilic cell compartment in one case and within the early polychromatic cell compartment in all 6 cases. The results indicate the existence of out-of-cycle cells, which may be responsible for the inefficacy for erythropoiesis in this disease. The defect was present in all 4 cases with acute erythroleukaemia. In one of the two cases showing no deterioration of the physical and haematological condition over a period of years, an additional defect was observed, consisting of a striking accumulation of tetraploid unlabelled cells. Therefore, the technique used may be suitable as a diagnostic tool for evaluation of new types of ineffective erythropoiesis.
在最初表现为红白血病症状的6例患者中，通过体内用3H-TdR标记后联合福尔根细胞光度法和放射自显影术研究了成红细胞的增殖情况。观察到一种增殖紊乱，表现为二倍体和未标记细胞的积累以及处于S期的细胞比例降低。这种缺陷在1例患者的嗜碱性细胞区室中出现，在所有6例患者的早期多色性细胞区室中均出现。结果表明存在细胞周期外细胞，这可能是该疾病红细胞生成无效的原因。在所有4例急性红白血病患者中均存在这种缺陷。在两例多年来身体和血液学状况未恶化的患者中，观察到另一种缺陷，即四倍体未标记细胞显著积累。因此，所使用的技术可能适合作为评估新型无效红细胞生成的诊断工具。
