Mitrou P S, Fischer M, Hübner K
Acta Haematol. 1975;54(5):271-9. doi: 10.1159/000208085.
Erythropoiesis of two patients in the early stage of acute leukaemia and two patients with refractory anaemia and hypercellular bone marrow (preleukaemia?) was studied with the cytophotometric-autoradiographic method. Megaloblastoid erythroblasts show a decreased proliferative activity in comparison to the morphologically normal cells and are in the maturation stage of E4 (early polychromatic normoblasts) mainly in the G1-phase; they are therefore largely comparable to the megaloblastoid erythroblasts in erythroleukaemia. Erythroblasts in preleukaemia with nuclear abnormalities are found in a high percentage in the G2-phase or are unlabelled with a DNA content of between diploid and tetraploid value. They show a similar proliferative behaviour to the megaloblasts in pernicious anaemia. Early polychromatic erythroblasts arrested in G2-phase can differentiate without mitosis into tetraploid mature erythroblasts (E5). They can divide elsewhere endomitotically, produce binucleated E5 or take up the DNA synthesis and become polyploid. E4 with nuclear abnormalities do not proliferate and are mainly found in the premitotic phase.
采用细胞光度 - 放射自显影法对两名急性白血病早期患者以及两名难治性贫血伴骨髓细胞增多（白血病前期？）患者的红细胞生成进行了研究。与形态学正常的细胞相比，巨幼样幼红细胞的增殖活性降低，主要处于E4期（早幼多染性正成红细胞）的成熟阶段，且主要处于G1期；因此，它们在很大程度上与红白血病中的巨幼样幼红细胞相似。白血病前期有核异常的幼红细胞在G2期的比例很高，或者DNA含量在二倍体和四倍体值之间且未被标记。它们表现出与恶性贫血中的巨幼细胞相似的增殖行为。停滞在G2期的早幼多染性红细胞可不经有丝分裂而分化为四倍体成熟红细胞（E5）。它们可在其他部位进行核内有丝分裂，产生双核E5，或进行DNA合成并成为多倍体。有核异常的E4不增殖，主要见于有丝分裂前期。
