Fu Qiuli, Qin Zhenwei, Yu Jiexin, Yu Yinhui, Tang Qiaomei, Lyu Danni, Zhang Lifang, Chen Zhijian, Yao Ke
Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University Zhejiang Provincial Key Lab of Ophthalmology The First Affiliated Hospital of Zhejiang Chinese Medical University, Zhejiang Provincial Hospital of TCM Department of Environmental and Occupational Health, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang Province, China.
Medicine (Baltimore). 2016 Jun;95(25):e3869. doi: 10.1097/MD.0000000000003869.
The aging of lens progenitor cell has been repeatedly proposed to play a key role in age-related cataracts (ARCs), but the mechanism is far from being understood. The present study aims to investigate the relationship between aging of lens progenitor/epithelial cells and the 4 subtypes of ARCs in humans.Lens capsules, which were collected from ARC patients during surgery, were divided into 3 groups according to the age of patients (50-60, 60-80, and >80 years). The expressions of lens progenitor cell-related markers Sox2, Abcg2, and Ki67 were first examined in human lens epithelial cells (HLECs) in situ. Then, the percentage of senescent and SA-β-gal HLECs isolated from lens capsules were quantified. Finally, the potential relationships between the percentage of senescent (and SA-β-gal) HLECs and the severity of ARCs were analyzed.Ki67, Sox2, and Abcg2 HLECs in lens capsules were clearly more abundant in young people than in patients older than 50 years, and they were almost absent in patients older than 60 years. The percentage of primary HLECs with aging morphology increased with age, consistent with the results of SA-β-gal primary HLECs. Only cortical cataract classification was found to be strongly related to the percentage of SA-β-gal and senescent HLECs.Our study gave the initial evidence on the dynamical change of lens stem/progenitor cells in human lens capsule with age and suggested that lens progenitor/epithelial cell aging is important in the severity of cortical cataracts.
晶状体祖细胞衰老在年龄相关性白内障（ARCs）中发挥关键作用的观点已被多次提出，但其机制仍远未明确。本研究旨在探讨晶状体祖细胞/上皮细胞衰老与人类ARCs 4种亚型之间的关系。在手术过程中从ARC患者收集的晶状体囊膜，根据患者年龄（50 - 60岁、60 - 80岁和>80岁）分为3组。首先在人晶状体上皮细胞（HLECs）原位检测晶状体祖细胞相关标志物Sox2、Abcg2和Ki67的表达。然后，对从晶状体囊膜分离的衰老和SA-β-gal HLECs的百分比进行定量。最后，分析衰老（和SA-β-gal）HLECs百分比与ARCs严重程度之间的潜在关系。晶状体囊膜中的Ki67、Sox2和Abcg2 HLECs在年轻人中明显比50岁以上患者丰富，在60岁以上患者中几乎不存在。具有衰老形态的原代HLECs百分比随年龄增加，与SA-β-gal原代HLECs结果一致。仅发现皮质性白内障分级与SA-β-gal和衰老HLECs百分比密切相关。我们的研究为人类晶状体囊膜中晶状体干/祖细胞随年龄的动态变化提供了初步证据，并表明晶状体祖细胞/上皮细胞衰老在皮质性白内障的严重程度中起重要作用。
