Chen Qifang, Gu Peilin, Liu Xuemei, Hu Shaohua, Zheng Hong, Liu Ting, Li Chongyi
Department of Ophthalmology, Daping Hospital, Army Medical University, Chongqing 400042, China.
Department of Thoracic Surgery, Xinqiao Hospital, Army Medical University, Chongqing 400037, China.
Pharmaceuticals (Basel). 2022 Dec 25;16(1):26. doi: 10.3390/ph16010026.
Nanoparticle-based drug delivery systems, which can overcome the challenges associated with poor aqueous solubility and other harmful side effects of drugs, display potent applications in cataract treatment. Herein, we designed a nanosystem of gold nanoparticles containing resveratrol (RGNPs) as an anti-aging agent to delay cataracts. The spherical RGNPs had a superior ability to inhibit hydrogen peroxide-mediated oxidative stress damage, including reactive oxygen species (ROS) production, malondialdehyde (MDA) generation, and glutathione (GSH) consumption in the lens epithelial cells. Additionally, the present data showed that RGNPs could delay cellular senescence induced by oxidative stress by decreasing the protein levels of p16 and p21, reducing the ratio of BAX/BCL-2 and the senescence-associated secretory phenotype (SASP) in vitro. Moreover, the RGNPs could also clearly relieve sodium selenite-induced lens opacity in a rat cataract model. Our data indicated that cell senescence was reduced and cataracts were delayed upon treatment with RGNPs through activating the Sirt1/Nrf2 signaling pathway. Our findings suggested that RGNPs could serve as an anti-aging ingredient, highlighting their potential to delay cataract development.
基于纳米颗粒的药物递送系统能够克服与药物水溶性差及其他有害副作用相关的挑战，在白内障治疗中显示出强大的应用潜力。在此，我们设计了一种含有白藜芦醇的金纳米颗粒纳米系统（RGNPs）作为抗衰老剂来延缓白内障。球形RGNPs具有卓越的抑制过氧化氢介导的氧化应激损伤的能力，包括晶状体上皮细胞中活性氧（ROS）的产生、丙二醛（MDA）的生成以及谷胱甘肽（GSH）的消耗。此外，目前的数据表明，RGNPs可通过降低p16和p21的蛋白水平、降低BAX/BCL-2的比值以及体外衰老相关分泌表型（SASP），来延缓氧化应激诱导的细胞衰老。此外，RGNPs还能在大鼠白内障模型中明显减轻亚硒酸钠诱导的晶状体混浊。我们的数据表明，通过激活Sirt1/Nrf2信号通路，用RGNPs治疗可减少细胞衰老并延缓白内障。我们的研究结果表明，RGNPs可作为一种抗衰老成分，突出了其延缓白内障发展的潜力。
