Lefrançois D, al Achkar W, Aurias A, Couturier J, Dutrillaux A M, Dutrillaux B, Flüry-Herard A, Gerbault-Seureau M, Hoffschir F, Lamoliatte E
U.R.A. 620 C.N.R.S., Institut Curie, Section de Biologie, Paris, France.
Mutat Res. 1989 Jun;212(2):167-72. doi: 10.1016/0027-5107(89)90068-7.
The effect of low-dose (0-0.5 Gy) gamma-radiations was studied on R-banded chromosomes from lymphocytes of healthy donors of various ages. In cells from newborns, an increase of chromosome damage roughly proportional to the dose was found. In lymphocytes from young adults chromosomal aberrations were not detected at doses of 0.05 and 0.1 Gy, and in lymphocytes from old adults chromosomal aberrations were not detected at doses of 0.05 and 0.1 Gy, and in lymphocytes from old adults not even at 0.2 Gy. The difficulty in detecting aberrations in lymphocytes from adults is largely due to a considerable background of chromosomal anomalies which should be borne in mind in dosimetry studies. The rate of induction largely depends on the types of rearrangements. One-break terminal deletions are efficiently induced at 0.1 and 0.2 Gy and are the best indicators of exposure at these doses. At 0.5 Gy, the frequencies of 2-break lesions, i.e., dicentrics and reciprocal translocations, increase, whereas that of deletions decreases.
研究了低剂量（0 - 0.5戈瑞）γ辐射对不同年龄健康供体淋巴细胞R带染色体的影响。在新生儿的细胞中，发现染色体损伤的增加大致与剂量成正比。在年轻成年人的淋巴细胞中，0.05和0.1戈瑞剂量下未检测到染色体畸变；在老年人的淋巴细胞中，0.05和0.1戈瑞剂量下未检测到染色体畸变，甚至在0.2戈瑞剂量下也未检测到。在成年人淋巴细胞中检测畸变的困难很大程度上是由于染色体异常的相当大的背景，这在剂量学研究中应予以考虑。诱导率在很大程度上取决于重排的类型。单断点末端缺失在0.1和0.2戈瑞时能有效诱导，是这些剂量下暴露的最佳指标。在0.5戈瑞时，双断点损伤（即双着丝粒体和相互易位）的频率增加，而缺失的频率降低。
