Chen Zhihong, Huang Guilin, Zhang Nini, Yi Jie, Yao Li, Zhang Lin
Hua Xi Kou Qiang Yi Xue Za Zhi. 2016 Apr;34(2):178-82. doi: 10.7518/hxkq.2016.02.015.
To explore the effects of aspirin and inflammation on the maturation and function of dendritic cells (DC) on the supernatant of VX-2 squamous cell carcinoma.
The rabbit buccal VX-2 squamous cell carcinoma models with inflammation were established by tumor particle implantation, mechanical trauma, and high sugar diet. The rabbits were divided into three groups. For the experimental group (rabbit buccal VX-2 squamous cell carcinoma with local inflammation), aspirin were given by gavage for three consecutive days. For the control group (rabbit buccal VX-2 squamous cell carcinoma with local inflammation), normal saline was given by gavage for three consecutive days. For the blank group (tumor without inflammation), normal saline was given by gavage for three consecutive days. Each tumor specimens were collected in three days and made into tissue homogenate. The supernatant was collected after centrifugation. Normal rabbit peripheral blood mononuclear cells were separated and co-cultured with different states of supernatant. The expression of DC surface markers CD83, CD86, and human leukocyte antigen-DR (HLA-DR) were detected by flow cytometry. The state of function of DC was tested by mixed lymphocyte reaction.
The positive rate of CD83, CD86, and HLA-DR of the experimental and control groups were both lower than that of the blank group (P<0.05). In addition, the ability to stimulate T cell proliferation of the experimental and control groups were weaker than that of the blank group (P<0.05). No significant difference was observed between the experi- and HLADR of DC. The short-term administration of aspirin is not conducive to the phenoty and function of DC in a rabbit mental and control groups (P>0.05).
Inflammation may inhibit the function and expression of CD83, CD86, buccal VX-2 squamous cell carcinoma inflammatory environment
探讨阿司匹林和炎症对VX-2鳞状细胞癌上清液中树突状细胞（DC）成熟及功能的影响。
通过肿瘤颗粒植入、机械创伤和高糖饮食建立伴有炎症的兔颊部VX-2鳞状细胞癌模型。将兔分为三组。实验组（兔颊部伴有局部炎症的VX-2鳞状细胞癌）连续三天灌胃给予阿司匹林。对照组（兔颊部伴有局部炎症的VX-2鳞状细胞癌）连续三天灌胃给予生理盐水。空白组（无炎症的肿瘤）连续三天灌胃给予生理盐水。三天后收集各肿瘤标本并制成组织匀浆。离心后收集上清液。分离正常兔外周血单个核细胞并与不同状态的上清液共培养。采用流式细胞术检测DC表面标志物CD83、CD86和人类白细胞抗原-DR（HLA-DR）的表达。通过混合淋巴细胞反应检测DC的功能状态。
实验组和对照组CD83、CD86和HLA-DR的阳性率均低于空白组（P<0.05）。此外，实验组和对照组刺激T细胞增殖的能力均弱于空白组（P<0.05）。实验组和对照组之间DC的表型和功能无显著差异（P>0.05）。短期给予阿司匹林不利于兔颊部VX-2鳞状细胞癌炎症环境中DC的表型和功能。
炎症可能抑制CD83、CD86的功能和表达。
