Shippen-Lentz D E, Vezza A C
Department of Biology, University College, University of Alabama at Birmingham 35294.
Mol Biochem Parasitol. 1988 Jan 15;27(2-3):263-73. doi: 10.1016/0166-6851(88)90046-1.
5S rRNA and rDNA from Plasmodium falciparum have been characterized. The 5S rRNA transcripts isolated from erythrocytic stage parasites are composed of three distinct subclasses, 117-119 nucleotides in length, which are identical in sequence with the exception of one or two additional uridine residues at the 3' terminus. Southern blot analysis of genomic DNA identified three 5S rRNA gene classes which are clustered within 1.5 kb of DNA. Cloning and sequence analyses of the 5S rDNA revealed identical coding regions surrounded by divergent extremely A+T rich flanking sequences (greater than 90%). Typical PolIII termination signals (6-8) T residues abut each coding region. Copy number analysis indicates that P. falciparum contains only three 5S rRNA genes, the lowest number reported for any organism.
恶性疟原虫的5S rRNA和rDNA已被鉴定。从红细胞期寄生虫中分离出的5S rRNA转录本由三个不同的亚类组成，长度为117 - 119个核苷酸，除了3'末端有一两个额外的尿苷残基外，序列相同。基因组DNA的Southern印迹分析确定了三个5S rRNA基因类，它们聚集在1.5 kb的DNA范围内。5S rDNA的克隆和序列分析显示，相同的编码区域被不同的富含A + T的侧翼序列（大于90%）包围。典型的PolIII终止信号（6 - 8个）T残基邻接每个编码区域。拷贝数分析表明，恶性疟原虫仅含有三个5S rRNA基因，这是任何生物体中报道的最低数量。
