State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072,China.
University of Chinese Academy of Sciences, Beijing 100039, China.
Sci Rep. 2016 Jun 27;6:28679. doi: 10.1038/srep28679.
Growth related traits in fish are controlled by quantitative trait loci (QTL), but no QTL for growth have been detected in bighead carp (Hypophthalmichthys nobilis) due to the lack of high-density genetic map. In this study, an ultra-high density genetic map was constructed with 3,121 SNP markers by sequencing 117 individuals in a F1 family using 2b-RAD technology. The total length of the map was 2341.27 cM, with an average marker interval of 0.75 cM. A high level of genomic synteny between our map and zebrafish was detected. Based on this genetic map, one genome-wide significant and 37 suggestive QTL for five growth-related traits were identified in 6 linkage groups (i.e. LG3, LG11, LG15, LG18, LG19, LG22). The phenotypic variance explained (PVE) by these QTL varied from 15.4% to 38.2%. Marker within the significant QTL region was surrounded by CRP1 and CRP2, which played an important role in muscle cell division. These high-density map and QTL information provided a solid base for QTL fine mapping and comparative genomics in bighead carp.
鱼类的生长相关性状受数量性状位点（QTL）控制，但由于缺乏高密度遗传图谱，大头鱼（Hypophthalmichthys nobilis）的生长 QTL 尚未被检测到。本研究采用 2b-RAD 技术对 117 个 F1 个体进行测序，构建了一张包含 3121 个 SNP 标记的超高密度遗传图谱。图谱总长度为 2341.27cM，平均标记间隔为 0.75cM。检测到本图谱与斑马鱼之间存在高度的基因组同线性。基于该遗传图谱，在 6 个连锁群（即 LG3、LG11、LG15、LG18、LG19 和 LG22）中鉴定出了 5 个生长相关性状的 1 个全基因组显著和 37 个提示性 QTL。这些 QTL 解释的表型方差（PVE）从 15.4%到 38.2%不等。显著 QTL 区域内的标记被 CRP1 和 CRP2 包围，CRP1 和 CRP2 在肌肉细胞分裂中起重要作用。这些高密度图谱和 QTL 信息为大头鱼的 QTL 精细定位和比较基因组学提供了坚实的基础。
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