Hu Xingyou, Sheng Jiaoyue, Guan Guoping, Ju Tongzhong, Smith David F, Wang Lu
College of Textiles & Clothing, Qingdao University, Qingdao 266000, China.
Department of Technical Textiles, College of Textiles, Donghua University, Shanghai 201620, China.
J Funct Biomater. 2022 Nov 11;13(4):235. doi: 10.3390/jfb13040235.
Biomaterials have been widely used as substitutes for diseased tissue in surgery and have gained great success and attention. At present, the biocompatibility of biomaterials such as PET woven fabrics is often evaluated both in vitro and in vivo. However, the current experimental methods cannot reveal the relationship between material surfaces and cell adhesion, and few research works have focused on the mechanisms of how the surface morphology of biomaterials affects cell adhesion and proliferation. Thus, it is meaningful to find out how the altered surfaces could affect cell adhesion and growth. In this study, we employed Ar low-temperature plasma treatment technology to create nano-grooves on the warp yarn of PET woven fabrics and seeded human umbellar vein endothelial cells (HUVEC) on these fabrics. We then assessed the O-glycan and N-glycan profiles of the cells grown on different structures of the polyester woven fabrics. The result showed that the surface morphology of polyester woven fabrics could affect the O-glycan profile but not the N-glycan profile of cultured HUVEC. Taken together, the study describes the effects of the surface morphology of biomaterial on the biosynthesis of cellular glycans and may provide new insights into the design and manufacture of biomaterials used as blood vessels based on the expression profiles of O-glycans on cultured cells.
生物材料已被广泛用作外科手术中病变组织的替代品，并取得了巨大成功且备受关注。目前，聚对苯二甲酸乙二酯（PET）机织织物等生物材料的生物相容性通常在体外和体内进行评估。然而，当前的实验方法无法揭示材料表面与细胞黏附之间的关系，很少有研究工作关注生物材料的表面形态如何影响细胞黏附和增殖的机制。因此，弄清楚表面改变如何影响细胞黏附和生长具有重要意义。在本研究中，我们采用氩低温等离子体处理技术在PET机织织物的经纱上制造纳米凹槽，并在这些织物上接种人脐静脉内皮细胞（HUVEC）。然后，我们评估了在聚酯机织织物不同结构上生长的细胞的O-聚糖和N-聚糖谱。结果表明，聚酯机织织物的表面形态会影响培养的HUVEC的O-聚糖谱，但不会影响其N-聚糖谱。综上所述，该研究描述了生物材料表面形态对细胞聚糖生物合成的影响，并可能基于培养细胞上O-聚糖的表达谱，为用作血管的生物材料的设计和制造提供新的见解。
