Kai Masanori, Fafutis-Morris Mary, Miyamoto Yuji, Mukai Tetsu, Mayorga-Rodriguez Jorge, Rodriguez-Castellanos Marco Antonio, Martínez-Guzman Marco Alonso, Matsuoka Masanori
Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
University of Guadalajara/Instituto Dermatologico de Jalisco, Guadalajara, Jalisco, Mexico.
J Dermatol. 2016 Nov;43(11):1345-1349. doi: 10.1111/1346-8138.13483. Epub 2016 Jun 27.
Mycobacterium lepromatosis, an independent species from Mycobacterium leprae, has been found to be a causative agent for diffuse lepromatous leprosy (DLL) in Mexico, but remains poorly studied. Here, the drug resistance-determining regions (DRDR) of folP1, rpoB and gyrA (conferring resistance to dapsone, rifampicin and quinolone, respectively) in M. lepromatosis from leprosy patients in Mexico were characterized. No mutations or silent mutations were found at previously characterized major sites in DRDR of M. lepromatosis. However, a non-synonymous mutation was found in codon 54 between two major sites of the folP1 DRDR in M. lepromatosis sequences. All M. lepromatosis isolates showed CAG sequence in codon 54 of folP1. Because the next codons 53 and 55 are known as major mutation sites for drug resistance, more detailed analysis using more samples is needed to determine whether it influences susceptibility to dapsone and/or efficiency of folate biosynthesis in M. lepromatosis or not.
麻风分枝杆菌是一种独立于麻风杆菌的菌种，已被发现是墨西哥弥漫性瘤型麻风（DLL）的病原体，但对其研究仍然较少。在此，对来自墨西哥麻风患者的麻风分枝杆菌中folP1、rpoB和gyrA的耐药性决定区域（DRDR）（分别赋予对氨苯砜、利福平和喹诺酮的耐药性）进行了特征分析。在麻风分枝杆菌DRDR先前确定的主要位点未发现突变或沉默突变。然而，在麻风分枝杆菌序列中folP1 DRDR的两个主要位点之间的第54密码子发现了一个非同义突变。所有麻风分枝杆菌分离株在folP1的第54密码子均显示CAG序列。由于接下来的第53和55密码子是已知的耐药主要突变位点，因此需要使用更多样本进行更详细的分析，以确定其是否影响麻风分枝杆菌对氨苯砜的敏感性和/或叶酸生物合成效率。
