Dumevska Biljana, Bosman Alexis, McKernan Robert, Goel Divya, Peura Teija, Schmidt Uli
Genea Biocells, Sydney, Australia.
Stem Cell Res. 2016 Jan;16(1):155-8. doi: 10.1016/j.scr.2015.10.002. Epub 2015 Oct 9.
The Genea002 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XY karyotype by CGH and male Allele pattern through STR analysis. Pluripotency of Genea002 was demonstrated with 75% of cells expressing Nanog, 93% Oct4, 83% Tra1-60 and 98% SSEA4, a Pluritest pluripotency score of 24.55, Novelty score of 1.39, teratomas with tissues from all embryonic germ layers and Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and any visible contamination.
Genea002人胚胎干细胞系源自一个捐赠的、已获得完全商业同意的辅助生殖技术囊胚，通过在灭活饲养层上培养内细胞团生长而来。该细胞系呈现多能干细胞形态，基因组分析通过比较基因组杂交验证为46, XY核型，并通过短串联重复序列分析确定为男性等位基因模式。Genea002的多能性表现为75%的细胞表达Nanog、93%表达Oct4、83%表达Tra1-60、98%表达SSEA4，Pluritest多能性评分为24.55，新颖性评分为1.39，能形成包含所有胚胎胚层组织的畸胎瘤且具有碱性磷酸酶活性。该细胞系支原体检测为阴性，无任何可见污染。
