Dumevska Biljana, Bosman Alexis, McKernan Robert, Goel Divya, Schmidt Uli, Peura Teija
Genea Biocells, Sydney, Australia.
Genea Biocells, Sydney, Australia.
Stem Cell Res. 2016 Mar;16(2):456-9. doi: 10.1016/j.scr.2016.02.012. Epub 2016 Feb 3.
The Genea023 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XY karyotype and male allele pattern through CGH and STR analysis. Pluripotency of Genea023 was demonstrated with 85% of cells expressed Nanog, 98% Oct4, 55% Tra1-60 and 98% SSEA4, gave a Pluritest Pluripotency score of 42.76, Novelty of 1.23, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
Genea023人胚胎干细胞系源自一个捐赠的、已获得完全商业同意的辅助生殖技术囊胚，通过在灭活饲养层上培养内细胞团生长而来。该细胞系呈现多能干细胞形态，基因组分析通过比较基因组杂交（CGH）和短串联重复序列（STR）分析验证了46, XY核型和男性等位基因模式。Genea023的多能性表现为85%的细胞表达Nanog、98%表达Oct4、55%表达Tra1-60以及98%表达阶段特异性胚胎抗原4（SSEA4），Pluritest多能性评分为42.76，新颖性评分为1.23，显示出碱性磷酸酶活性并形成三胚层畸胎瘤。该细胞系支原体检测呈阴性且无可见污染。
