Dumevska Biljana, Chami Omar, McKernan Robert, Goel Divya, Schmidt Uli
Genea Biocells, Sydney, Australia.
Genea Biocells, Sydney, Australia.
Stem Cell Res. 2016 Mar;16(2):322-6. doi: 10.1016/j.scr.2015.11.012. Epub 2015 Nov 26.
The Genea047 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated human feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype and female allele pattern through traditional karyotyping, CGH and STR analysis. Pluripotency of Genea047 was demonstrated with 88% of cells expressing Nanog, 95% Oct4, 59% Tra1-60 and 99% SSEA4, a PluriTest Pluripotency score of 30.86, Novelty score of 1.23 and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and any visible contamination.
Genea047人胚胎干细胞系源自一个捐赠的、已获得完全商业同意的辅助生殖技术囊胚，通过在灭活的人饲养层上进行内细胞团生长培养获得。该细胞系呈现多能细胞形态，基因组分析通过传统核型分析、比较基因组杂交（CGH）和短串联重复序列（STR）分析，证实了其46, XX核型和女性等位基因模式。Genea047的多能性通过以下方面得以证明：88%的细胞表达Nanog，95%表达Oct4，59%表达Tra1-60，99%表达阶段特异性胚胎抗原4（SSEA4），多能性检测PluriTest评分为30.86，新颖性评分为1.23，且能形成三胚层畸胎瘤。该细胞系支原体检测呈阴性，无任何可见污染。
