人类胚胎干细胞系Genea022的衍生
Derivation of human embryonic stem cell line Genea022.
作者信息
Dumevska Biljana, Bosman Alexis, McKernan Robert, Schmidt Uli, Peura Teija
机构信息
Genea Biocells, Sydney, Australia.
出版信息
Stem Cell Res. 2016 Mar;16(2):472-5. doi: 10.1016/j.scr.2016.02.011. Epub 2016 Feb 11.
The Genea022 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XY karyotype and male allele pattern through CGH and STR analysis. Pluripotency of Genea022 was demonstrated with 84% of cells expressed Nanog, 98% Oct4, 55% Tra1-60 and 97% SSEA4, gave a Pluritest Pluripotency score of 42.95, Novelty of 1.23, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
Genea022人胚胎干细胞系源自一个捐赠的、已获得完全商业同意的辅助生殖技术囊胚，通过在灭活饲养层上进行内细胞团生长培养获得。该细胞系呈现多能干细胞形态，基因组分析通过比较基因组杂交（CGH）和短串联重复序列（STR）分析，证实其核型为46, XY且具有男性等位基因模式。Genea022的多能性表现为84%的细胞表达Nanog，98%表达Oct4，55%表达Tra1-60，97%表达阶段特异性胚胎抗原4（SSEA4），Pluritest多能性评分为42.95，新颖性评分为1.23，具有碱性磷酸酶活性并能形成三胚层畸胎瘤。该细胞系支原体检测为阴性且无可见污染。
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