人类胚胎干细胞系Genea019的衍生
Derivation of human embryonic stem cell line Genea019.
作者信息
Dumevska Biljana, Peura Teija, McKernan Robert, Goel Divya, Schmidt Uli
机构信息
Genea Biocells, Sydney, Australia.
出版信息
Stem Cell Res. 2016 Mar;16(2):397-400. doi: 10.1016/j.scr.2016.02.008. Epub 2016 Feb 3.
The Genea019 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype, female Allele pattern and unaffected Htt CAG repeat length, compared to HD affected sibling Genea020. Pluripotency of Genea019 was demonstrated with 75% of cells expressing Nanog, 89% Oct4, 48% Tra1-60 and 85% SSEA4, a Pluritest Pluripotency score of 22.97, Novelty score of 1.42, tri-lineage teratoma formation and Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and any visible contamination.
Genea019人胚胎干细胞系源自一个捐赠的、已获得完全商业同意的辅助生殖技术囊胚，通过在灭活饲养层上培养内细胞团生长而来。与亨廷顿舞蹈病（HD）受累的同胞细胞系Genea020相比，该细胞系呈现多能细胞形态，基因组分析证实其核型为46, XX，具有女性等位基因模式且亨廷顿蛋白（Htt）CAG重复长度未受影响。Genea019的多能性表现为75%的细胞表达Nanog、89%表达Oct4、48%表达Tra1-60、85%表达阶段特异性胚胎抗原4（SSEA4），多能性测试（Pluritest）的多能性评分为22.97，新颖性评分为1.42，能形成三胚层畸胎瘤并具有碱性磷酸酶活性。该细胞系支原体检测呈阴性，且无任何可见污染。
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