Nogueira D M, Cavalieri J, Fitzpatrick L A, Gummow B, Blache D, Parker A J
Embrapa Semiárido, BR 428, Km 152. Caixa Postal 23, CEP 56302-970, Petrolina, PE, Brazil; College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, QLD 4811, Australia.
College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, QLD 4811, Australia.
Anim Reprod Sci. 2016 Aug;171:87-97. doi: 10.1016/j.anireprosci.2016.06.003. Epub 2016 Jun 15.
This study aimed to evaluate the reproductive response of anoestrous goats that were either hormonally treated and/or supplemented with maize for 9days to determine which treatment combination was the most effective in enhancing follicular development and ovulation rate, and whether these responses were associated with increases in metabolic hormones. The experiment was carried out using 28 does, using a 2×2 factorial design with seven does in each group to test the effect of synchronisation of oestrus, supplementation with maize and their interactions. Synchronisation of oestrous cycles (P<0.001) but not supplementation with maize or the interaction between the two (P>0.05) increased the number of codominant follicles, the diameter of the largest follicle on Day 9 and growth rate of follicles during the period of supplementation. Compared with non-supplemented animals, supplementation with maize increased the total number of follicles observed between Days 7 and 9 (P=0.039). In addition, nutritional supplementation with maize in combination with synchronisation of oestrus increased the ovulation rate by 43% (P=0.074). Interactions between time and supplementation with maize showed that plasma concentrations of insulin, leptin and IGF-1 were greater in does supplemented with maize compared with non-supplemented does (P<0.001). The findings show that hormonal synchronisation had the most influence on modifying follicular development and ovulation in anoestrous goats. Supplementation with maize increased the concentrations of insulin, leptin and IGF-1, which could potentially modify the sensitivity of follicles to gonadotrophins and reduce rates of atresia.
本研究旨在评估对处于乏情期的山羊进行激素处理和/或补充玉米9天的繁殖反应，以确定哪种处理组合在促进卵泡发育和排卵率方面最有效，以及这些反应是否与代谢激素的增加有关。该实验使用了28只母羊，采用2×2析因设计，每组7只母羊，以测试发情同步、玉米补充及其相互作用的效果。发情周期同步（P<0.001）可增加优势卵泡数量、第9天最大卵泡直径以及补充期卵泡生长速度，但玉米补充或二者之间的相互作用并无此效果（P>0.05）。与未补充玉米的动物相比，补充玉米可增加第7至9天观察到的卵泡总数（P=0.039）。此外，玉米营养补充与发情同步相结合可使排卵率提高43%（P=0.074）。时间与玉米补充之间的相互作用表明，与未补充玉米的母羊相比，补充玉米的母羊血浆中胰岛素、瘦素和IGF-1浓度更高（P<0.001）。研究结果表明，激素同步对乏情期山羊卵泡发育和排卵的影响最大。补充玉米可提高胰岛素、瘦素和IGF-1的浓度，这可能会改变卵泡对促性腺激素的敏感性并降低闭锁率。
