Nogueira Daniel M, Eshtaeba Ahmad, Cavalieri John, Fitzpatrick Lee A, Gummow Bruce, Blache Dominique, Parker Anthony J
Animal Production and Reproduction, Embrapa Semiárido, Petrolina, Brazil; College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, Australia.
College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, Australia.
Theriogenology. 2017 Feb;89:97-105. doi: 10.1016/j.theriogenology.2016.10.014. Epub 2016 Oct 20.
This study aimed to evaluate ovarian follicular dynamics in goats submitted to synchronization of estrus and supplemented with diets that differed in the metabolizable energy source and amount of energy. The experiment was carried out using 42 does allocated into three treatments, fed for 9 days with a ration providing 1.0 times maintenance containing maize (1 MM, n = 14) or without maize (1 M, n = 14) or a ration providing 1.5 times maintenance containing maize (1.5 MM, n = 14). Estrus was synchronized with two injections of cloprostenol given 7 days apart. Does were also treated with intravaginal progesterone inserts and eCG. The number of ovulations and size of the follicles were measured using ultrasonography on Days 10, 11, and 12 after the start of the dietary treatment. The interval to estrus and duration of estrus did not differ between treatments (P = 0.382). Does fed with 1 and 1.5 MM had a similar number of ovulations but a greater number of ovulations than goats fed with 1 M (P = 0.028). The mean number of small, medium, large, and total number of follicles on Days 10 to 12 of ultrasound evaluations did not differ (P = 0.204) between treatments, but mean numbers changed over time (P < 0.001). The mean frequency and amplitude of LH pulses and concentrations of glucose, insulin, leptin, and insulin growth factor-1 in plasma were not significantly affected (P > 0.258) by any of the treatments. In summary, the inclusion of maize in the ration can stimulate ovulation rate at maintenance level. Similar results between groups fed diets that included maize and provided metabolizable energy at 1.0 and 1.5 maintenance demonstrate that to increase the ovulation rate when synchronizing estrous cycles in does, dietary supplementation with maize can be restricted to provide a maintenance level of metabolizable energy only, which would reduce dietary costs.
本研究旨在评估接受发情同步处理并补充不同代谢能来源和能量水平日粮的山羊的卵泡动态。实验使用了42只母羊，分为三种处理组，分别用提供维持能量1.0倍且含玉米的日粮（1MM，n = 14）、不含玉米的日粮（1M，n = 14）或提供维持能量1.5倍且含玉米的日粮（1.5MM，n = 14）喂养9天。发情通过间隔7天注射两次氯前列醇来同步。母羊还接受了阴道孕酮植入物和eCG处理。在日粮处理开始后的第10、11和12天，使用超声测量排卵数和卵泡大小。各处理组之间发情间隔和发情持续时间无差异（P = 0.382）。饲喂1MM和1.5MM的母羊排卵数相似，但比饲喂1M的山羊排卵数更多（P = 0.028）。超声评估第10至12天，各处理组中小卵泡、中卵泡、大卵泡的平均数量及卵泡总数无差异（P = 0.204），但平均数量随时间变化（P < 0.001）。任何处理对血浆中LH脉冲的平均频率和幅度以及葡萄糖、胰岛素、瘦素和胰岛素生长因子-1的浓度均无显著影响（P > 0.258）。总之，日粮中添加玉米可在维持水平刺激排卵率。在提供1.0和1.5倍维持代谢能且含玉米的日粮组之间得到的相似结果表明，在同步母羊发情周期时，若要提高排卵率，日粮中玉米的添加可仅限制在提供维持水平的代谢能，这将降低日粮成本。
