Alves Juliana Paula Martins, Fernandes César Carneiro Linhares, Rossetto Rafael, Silva Caroline Pessoa da, Galvão Iolly Tábata Oliveira Marques, Bertolini Marcelo, Rondina Davide
School of Veterinary Medicine, Ceará State University (UECE), Fortaleza, Brazil.
School of Veterinary Medicine, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
Reprod Domest Anim. 2019 Sep;54(9):1206-1216. doi: 10.1111/rda.13500. Epub 2019 Jul 25.
The aim of this study was to verify the effect of the energy source for a short-term diet supplementation on follicular dynamics, ovarian response and oocyte recovery in goats. Thirty Anglo Nubian crossbred does received a diet for 4 weeks to satisfy the nutritional requirements of breeding for adult non-dairy goats. Seven days prior to oocyte recovery (OR), a group of does (n = 10) was supplemented with ground full-fat linseed in the diet (Diet A), whereas a second group of does (n = 10) received crude glycerine in the diet (Diet B). The total mixed ration (TMR) diet was maintained as the Control Diet (n = 10). All animals were oestrous-synchronized by the use of a progesterone insert for 12 days prior to OR. Follicles were stimulated by using pFSH (five 40-mg/ml doses) during the supplementation time. At OR, follicles were counted and recovered oocytes were classified as viable or degenerated. Follicular dynamics was monitored by ultrasonography, and plasma glucose, cholesterol and triglyceride levels were measured during supplementation. Glucose was higher in Diet B and cholesterol in Diet A. Diet B had a lower proportion of small (<3 mm) and large follicles (≥3 mm; p = 0.01). The follicular growth rate was higher in Diet A (p < 0.01), with follicles emerging in the 5th day of supplementation. No differences were observed for follicles counted and oocytes recovered. Thus, the type of energy source supplemented for a short term was capable to alter the follicular dynamics, without affecting the proportion of morphologically viable oocytes upon recovery.
本研究的目的是验证短期日粮补充能量来源对山羊卵泡动态、卵巢反应和卵母细胞回收的影响。30只盎格鲁努比亚杂交母羊接受了为期4周的日粮，以满足成年非奶山羊繁殖的营养需求。在卵母细胞回收（OR）前7天，一组母羊（n = 10）在日粮中补充磨碎的全脂亚麻籽（日粮A），而另一组母羊（n = 10）在日粮中添加粗甘油（日粮B）。全混合日粮（TMR）作为对照日粮（n = 10）。在OR前12天，所有动物使用孕酮植入物进行发情同步。在补充期间，使用pFSH（五剂40mg/ml）刺激卵泡。在OR时，对卵泡进行计数，并将回收的卵母细胞分类为活的或退化的。通过超声监测卵泡动态，并在补充期间测量血浆葡萄糖、胆固醇和甘油三酯水平。日粮B中的葡萄糖较高，日粮A中的胆固醇较高。日粮B中小卵泡（<3mm）和大卵泡（≥3mm）的比例较低（p = 0.01）。日粮A中的卵泡生长率较高（p < 0.01），在补充的第5天出现卵泡。在计数的卵泡和回收的卵母细胞方面未观察到差异。因此，短期补充的能量来源类型能够改变卵泡动态，而不影响回收时形态学上活卵母细胞的比例。
