Chen Lih-Geeng, Su Pei-Jung, Tsai Po-Wei, Yang Ling-Ling, Wang Ching-Chiung
Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi, Taiwan.
Graduate Institute of Pharmacognosy Science, School of Pharmacy, Pharmacy of College, Taipei Medical University, Taipei, Taiwan.
Planta Med. 2017 Jan;83(1-02):151-157. doi: 10.1055/s-0042-109779. Epub 2016 Jun 28.
Eight ethanolic extracts of indigenous Taiwanese plants of the genus were tested for tumor cytotoxicity against AGS, Hep G2, HeLa, KB, and HL-60 cells. Among the 50 % and 95 % EtOH extracts of eight species, the cytotoxic effects of leaves were the strongest. When the leaf extract of was partitioned using -hexane and aqueous solvents, the -hexane layer showed a greater cytotoxic effect and could prolong the survival time of P-388D tumor-bearing CDF1 mice. Two new labdane diterpene derivatives, intermedin A () and intermedin B (), and coronarin E () were isolated from the -hexane layer of . Intermedin A induced apoptosis in HL-60 cells at 30 µg/mL and significantly prolonged the survival time of P-388D tumor-bearing CDF mice by 48.7 % at 20 mg/kg of body weight. We suggest that intermedin A is a major compound of and has a cytotoxic effect on HL-60 and P-388D cells.
