光动力疗法增强姜黄素对小儿上皮性肝肿瘤细胞的作用。
Photodynamic Therapy Potentiates the Effects of Curcumin on Pediatric Epithelial Liver Tumor Cells.
作者信息
Ellerkamp Verena, Bortel Nicola, Schmid Evi, Kirchner Bettina, Armeanu-Ebinger Sorin, Fuchs Jörg
机构信息
Department of Pediatric Surgery and Pediatric Urology, University Hospital Tuebingen, Tuebingen, Germany
Department of Pediatric Surgery and Pediatric Urology, University Hospital Tuebingen, Tuebingen, Germany.
出版信息
Anticancer Res. 2016 Jul;36(7):3363-72.
BACKGROUND/AIM: Curcumin (CUM) is a promising agent in complementary oncology. The present study analyzed the photoactive properties of curcumin on pediatric epithelial liver tumor cell lines.
MATERIALS AND METHODS
Hepatoblastoma cell lines (HuH6, HepT1) and hepatocellular carcinoma cell lines (HepG2, HC-AFW1) were treated with curcumin and exposed to blue light (phototherapy, 480 nm, 300 W). Cell viability (MTT tests), cellular oxidative stress (production of reactive oxygen species (ROS)) and cellular uptake/degradation of curcumin were analyzed.
RESULTS
Significant loss of viability resulted from 24-48 h incubation with curcumin. With photodynamic therapy (PDT), even short time incubation (1 h) with curcumin resulted in significantly lower half maximal inhibitory concentration (IC50) (p<0.001, two-way ANOVA). Significant ROS production was observed with PDT and curcumin.
CONCLUSION
Phototherapy strongly enhances the anticancer properties of curcumin in pediatric solid liver tumors in vitro.
背景/目的：姜黄素（CUM）是肿瘤辅助治疗中一种很有前景的药物。本研究分析了姜黄素对小儿上皮性肝肿瘤细胞系的光活性特性。
材料与方法
用姜黄素处理肝母细胞瘤细胞系（HuH6、HepT1）和肝细胞癌细胞系（HepG2、HC-AFW1），并使其暴露于蓝光下（光疗，480nm，300W）。分析细胞活力（MTT试验）、细胞氧化应激（活性氧（ROS）的产生）以及姜黄素的细胞摄取/降解情况。
结果
姜黄素孵育24 - 48小时导致细胞活力显著丧失。采用光动力疗法（PDT）时，即使姜黄素短时间孵育（1小时）也会使半数最大抑制浓度（IC50）显著降低（p<0.001，双向方差分析）。PDT联合姜黄素可观察到显著的ROS产生。
结论
光疗在体外可显著增强姜黄素对小儿实体肝肿瘤的抗癌特性。
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