Kang Yani, Li Hua, Wu Jun, Xu Xiaoting, Sun Xue, Zhao Xiaodong, Xu Nianjun
Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang, P.R. China.
School of Biomedical Engineering, Bio-ID Center, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, P.R. China.
PLoS One. 2016 Jun 29;11(6):e0158279. doi: 10.1371/journal.pone.0158279. eCollection 2016.
Seaweed is one of the important biomass producers and possesses active metabolites with potential therapeutic effects against tumors. The red alga Gracilariopsis lemaneiformis (Gp. lemaneiformis) possesses antitumor activity, and the polysaccharide of Gp. lemaneiformis (PGL) has been demonstrated to be an ingredient with marked anticancer activity. However, the anticancer mechanism of PGL remains to be elucidated. In this study, we analyzed the inhibitory effect of PGL on the cell growth of 3 human cancer cell lines and found that PGL inhibited cell proliferation, reduced cell viability, and altered cell morphology in a time- and concentration-dependent manner. Our transcriptome analysis indicates that PGL can regulate the expression of 758 genes, which are involved in apoptosis, the cell cycle, nuclear division, and cell death. Furthermore, we demonstrated that PGL induced apoptosis and cell cycle arrest and modulated the expression of related genes in the A549 cell line. Our work provides a framework to understand the effects of PGL on cancer cells, and can serve as a resource for delineating the antitumor mechanisms of Gp. lemaneiformis.
海藻是重要的生物质生产者之一，拥有具有潜在抗肿瘤治疗作用的活性代谢产物。红藻龙须菜具有抗肿瘤活性，龙须菜多糖（PGL）已被证明是一种具有显著抗癌活性的成分。然而，PGL的抗癌机制仍有待阐明。在本研究中，我们分析了PGL对3种人类癌细胞系细胞生长的抑制作用，发现PGL以时间和浓度依赖性方式抑制细胞增殖、降低细胞活力并改变细胞形态。我们的转录组分析表明，PGL可调节758个基因的表达，这些基因参与细胞凋亡、细胞周期、核分裂和细胞死亡。此外，我们证明PGL诱导A549细胞系凋亡和细胞周期停滞，并调节相关基因的表达。我们的工作为理解PGL对癌细胞的作用提供了一个框架，并可作为阐明龙须菜抗肿瘤机制的资源。
