Key Laboratory of Marine Genetics and Breeding ( Ocean University of China), Ministry of Education, 266003, Qingdao, People's Republic of China.
Mar Biotechnol (NY). 2023 Feb;25(1):100-108. doi: 10.1007/s10126-022-10184-2. Epub 2022 Dec 3.
ARTP (atmospheric and room temperature plasma mutagenesis) mutagenesis was tried on G. lemaneiformis, and mutagenesis conditions were confirmed. An osmotic pressure screening program was established. Mutants were identified and characterized of relevant physiological traits. The aim of the study is to try to use ARTP mutagenesis and osmotic pressure screening for the breeding of high-agar G. lemaneiformis. Treatment time of 46 s was found to be an optimal mutagenesis time. The mutagenized spores were initially screened with 58‰ salinity artificial seawater, and then, the surviving spores were screened twice with 60‰ salinity artificial seawater in their vertical growth phase and branch growth phase, respectively. Four fast-growing and hypertonic resistance gametophytes were selected. The actual photosynthetic efficiency [Y(PSII)], photochemical quenching (qL), and non-photochemical quenching (NPQ) of four mutants were measured. The values of Y(PSII) and qL of HAGL-X3 and HAGL-X5 were higher than those of the control in the early stage of salt stress. NPQs of HAGL-X3 and HAGL-X5 were higher than control in most of the times. The growth rates of the four mutants were higher than that of the control. HAGL-X4 was the highest. The agar content was measured; HAGL-X5 displayed the highest agar content among the tested strains. HAGL-X5 was more in line with expectations, because of its high agar content and good hypertonic resistance. In this study, the mutant of G. lemaneiformis with high agar content was obtained by the procedure, which provided a certain reference for the selection of G. lemaneiformis strains with high agar content.
ARTP（大气压室温等离子体诱变）诱变被应用于江蓠，确定了诱变条件。建立了渗透压筛选程序。对相关生理特性的突变体进行了鉴定和特征分析。本研究旨在尝试使用 ARTP 诱变和渗透压筛选进行高产琼脂江蓠的选育。发现 46 秒的处理时间是最佳的诱变时间。用 58‰盐度人工海水对诱变孢子进行初步筛选，然后在垂直生长阶段和分支生长阶段，用 60‰盐度人工海水对存活孢子进行两次筛选。筛选出 4 株生长迅速且耐高渗的配子体。测量了 4 株突变体的实际光合效率 [Y(PSII)]、光化学猝灭（qL）和非光化学猝灭（NPQ）。在盐胁迫的早期，HAGL-X3 和 HAGL-X5 的 Y(PSII)和 qL 值高于对照，而 HAGL-X3 和 HAGL-X5 的 NPQ 在大部分时间都高于对照。4 株突变体的生长速度均高于对照，其中 HAGL-X4 最高。测量了琼脂含量，HAGL-X5 表现出的琼脂含量高于其它测试菌株。HAGL-X5 更符合预期，因为它的琼脂含量高且耐高渗性好。本研究通过该程序获得了琼脂含量高的江蓠突变体，为选育琼脂含量高的江蓠菌株提供了一定的参考。
