Khinsar Kavish Hasnain, Abdul Sattar, Hussain Akbar, Ud Din Riaz, Lei Liu, Cao Jing, Abbasi Majid, Ur Rehman Ata, Farooqui Nabeel, Yi Xin, Min Huang, Wang Liang, Mintao Zhong
Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, 116044, China.
Department of Biochemistry, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning, 116044, China.
AMB Express. 2021 Dec 2;11(1):160. doi: 10.1186/s13568-021-01314-5.
Hepatocellular carcinoma is one of the leading causes of cancer-associated death across the globe. Malignant ascites are the major clinical attributes in cancer patients. Despite the advancements in HCC treatments such as chemotherapy, radiotherapy, surgery, and hormonal therapy, researchers are pursuing novel natural edible compounds for the treatment of cancer to eliminate dreadful side effects. Pleurotus ostreatus is one of the most edible cuisines in Asia as well as all over the world. It has been a source of nutritious diet since it was classified as an edible mushroom with no or negligible side effects. The present study focused on the natural anti-cancerous and anti-ascites capabilities of polysaccharides extracted from Pleurotus ostreatus in-vivo as well as in-vitro. Administration of polysaccharide Pleurotus ostreatus showed a significant decrease in tumor cell metastasis while the increase in the survival period among mice models of H22 malignant ascites. Downregulation of regenerative genes Foxp3 and Stat3 and secretion of immunological factors such as IL-2, TNF α, and INF γ were observed after treating with the partially pure extracted polysaccharide. Twining with the hypothesis of tumor suppression in-vivo model polysaccharide showed a decrease in invasion and migration abilities and henceforth responsible for the gene regulation such Cytochrome C which supposedly induced the chain of gene regulation process resulting in apoptosis in HCC cell lines observed in-vitro experiments. Collective research findings manifested that polysaccharide extracted from Pleurotus ostreatus bears anti-proliferative activity and thus influence tumor suppression in-vivo and in-vitro against hepatocellular carcinoma and can be used for therapeutic purposes as a potential anti-cancerous source in the future.
肝细胞癌是全球癌症相关死亡的主要原因之一。恶性腹水是癌症患者的主要临床特征。尽管肝癌治疗在化疗、放疗、手术和激素治疗等方面取得了进展，但研究人员仍在寻找新型天然可食用化合物来治疗癌症，以消除可怕的副作用。平菇是亚洲乃至全世界最常见的可食用菌类之一。自从它被归类为一种食用蘑菇且副作用极小或可忽略不计以来，它一直是营养饮食的来源。本研究聚焦于从平菇中提取的多糖在体内和体外的天然抗癌和抗腹水能力。给予平菇多糖后，肿瘤细胞转移显著减少，同时H22恶性腹水小鼠模型的生存期延长。在用部分纯化的提取多糖处理后，观察到再生基因Foxp3和Stat3的下调以及免疫因子如IL-2、TNFα和INFγ的分泌。与体内模型中肿瘤抑制的假设一致，多糖显示出侵袭和迁移能力的降低，因此负责基因调控，如细胞色素C，据推测它诱导了基因调控过程的连锁反应，导致体外实验中观察到的肝癌细胞系凋亡。综合研究结果表明，从平菇中提取的多糖具有抗增殖活性，因此在体内和体外对肝细胞癌都有抑制肿瘤的作用，未来可作为一种潜在的抗癌来源用于治疗目的。
