Makhzani Parastesh, Afghan Marjan, Tohidi Maryam, Bagheripour Fatemeh, Azizi Fereidoun, Ghasemi Asghar
a Faculty of Medicine , Shahid Beheshti University of Medical Sciences, Adjacent to Ayatollah Taleghani Hospital , Tehran , Iran.
b Endocrine Physiology Research Center, Research Institute for Endocrine Sciences , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
Endocr Res. 2017 May;42(2):96-101. doi: 10.1080/07435800.2016.1197933. Epub 2016 Jun 29.
It has been suggested that insulin resistance is associated with altered nitric oxide (NO) homeostasis. There is however no population-based study documenting an association between fasting serum insulin and serum NO metabolites (NOx) with multivariable adjustment. This study was therefore designed to determine the association between serum NOx and fasting insulin levels and insulin resistance/sensitivity indices in a sample of a population-based study in Iran.
This study, performed within the framework of the Tehran Lipid and Glucose Study (TLGS), analyzed the data of 1518 non-diabetic subjects (955 women), aged 20-87 years, who had participated in phase III of the TLGS (2006-2008). Serum NOx concentrations were measured using the Griess method. Fasting serum insulin was measured by the electrochemiluminescence immunoassay method. Multiple regression analysis was used to determine the association between serum NOx concentration and quartiles of insulin and insulin resistance/sensitivity indices (HOMA1-IR, HOMA2-IR, and QUICKI).
NOx concentration in women only was weakly correlated with HOMA1-IR (r = 0.07, P = 0.03) and QUICKI (r = -0.07, P = 0.03), whereas no significant association was observed in men (P > 0.05). Marginally significant correlations were also found between serum NOx and fasting insulin concentration (r = 0.062, p = 0.057) and HOMA2-IR (r = 0.063, p = 0.053) in women. NOx concentration differed significantly between quartiles of insulin and insulin resistance/sensitivity indices among women and the total population (P < 0.05), associations which remained significant after age adjustment (P < 0.05), but not after adjustment for other confounding variables (P > 0.05).
Fasting serum insulin level and insulin resistance/sensitivity indices are not associated with serum NOx level after multivariable adjustment.
有人提出胰岛素抵抗与一氧化氮（NO）内稳态改变有关。然而，尚无基于人群的研究记录空腹血清胰岛素与血清NO代谢产物（NOx）之间的关联并进行多变量调整。因此，本研究旨在确定在伊朗一项基于人群的研究样本中，血清NOx与空腹胰岛素水平以及胰岛素抵抗/敏感性指数之间的关联。
本研究在德黑兰血脂与血糖研究（TLGS）框架内进行，分析了1518名年龄在20 - 87岁的非糖尿病受试者（955名女性）的数据，这些受试者参与了TLGS的第三阶段（2006 - 2008年）。采用格里斯法测量血清NOx浓度。通过电化学发光免疫分析法测量空腹血清胰岛素。使用多元回归分析来确定血清NOx浓度与胰岛素四分位数以及胰岛素抵抗/敏感性指数（HOMA1 - IR、HOMA2 - IR和QUICKI）之间的关联。
仅在女性中，NOx浓度与HOMA1 - IR（r = 0.07，P = 0.03）和QUICKI（r = -0.07，P = 0.03）呈弱相关，而在男性中未观察到显著关联（P > 0.05）。在女性中，血清NOx与空腹胰岛素浓度（r = 0.062，p = 0.057）和HOMA2 - IR（r = 0.063，p = 0.053）之间也存在边缘显著相关性。女性和总人群中，胰岛素四分位数以及胰岛素抵抗/敏感性指数之间的NOx浓度存在显著差异（P < 0.05），年龄调整后这些关联仍然显著（P < 0.05），但在调整其他混杂变量后不显著（P > 0.05）。
多变量调整后，空腹血清胰岛素水平和胰岛素抵抗/敏感性指数与血清NOx水平无关。
