胰岛素样生长因子家族基因在肾透明细胞癌中的表达
Expression of insulin-like growth factor family genes in clear cell renal cell carcinoma.
作者信息
Braczkowski Ryszard, Białożyt Michał, Plato Marta, Mazurek Urszula, Braczkowska Bogumiła
机构信息
Department of Public Health, School of Public Health, Silesian Medical University, Katowice, Poland.
Prof. Michałowski Memory Hospital, Katowice, Poland.
出版信息
Contemp Oncol (Pozn). 2016;20(2):130-6. doi: 10.5114/wo.2016.58720. Epub 2016 Jun 14.
AIM OF THE STUDY
Despite significant progress in the pathology of clear cell renal cell carcinoma (ccRCC), diagnostic and predictive factors of major importance have not been discovered. Some hopes are associated with insulin-like growth factors. The aim of the study was to compare the expression of genes for insulin-like growth factor family in tumours and in tissue of kidneys without cancer.
MATERIAL AND METHODS
Fifty-two patients years with clear cell renal cell cancer were qualified to the study group; patients nephrectomised because of hydronephrosis were included in the control group. Expression of genes were evaluated by RT-PCR.
RESULTS
Expression of IGFR-1 gene in tumour accounts for about 60% of cases. The incidence is higher than in corresponding adjacent non-cancerous kidney tissues and higher (but with no statistical significance) than in kidney without cancer. Expression of IGFR-2 gene in tumours has not been established. The incidence of the expression in corresponding adjacent non-cancerous kidney tissues is small. Expression of this gene has been present in all specimens from kidneys without cancer. Expression of IGFBP-3 gene ascertained in all (except four) cases of ccRCC and in the majority of clippings from adjacent tissue. It was not found in kidneys from the control group. IGF-1, IGF-2, and IGFR-1 mRNA copy numbers in ccRCC were higher than in the material from the control group.
研究目的
尽管在透明细胞肾细胞癌（ccRCC）病理学方面取得了显著进展，但尚未发现具有重要意义的诊断和预测因素。胰岛素样生长因子带来了一些希望。本研究的目的是比较胰岛素样生长因子家族基因在肿瘤组织和无癌肾脏组织中的表达情况。
材料与方法
52例透明细胞肾细胞癌患者被纳入研究组；因肾积水接受肾切除术的患者被纳入对照组。通过逆转录聚合酶链反应（RT-PCR）评估基因表达。
结果
肿瘤组织中IGFR-1基因的表达在约60%的病例中存在。其发生率高于相应的相邻非癌性肾组织，且高于无癌肾脏组织（但无统计学意义）。肿瘤组织中未发现IGFR-2基因的表达。相应相邻非癌性肾组织中的表达发生率较低。该基因在所有无癌肾脏标本中均有表达。在所有（除4例）ccRCC病例及大多数相邻组织切片中均检测到IGFBP-3基因的表达。对照组肾脏中未发现该基因表达。ccRCC中IGF-1、IGF-2和IGFR-1 mRNA拷贝数高于对照组材料中的拷贝数。
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