基于量子点纳米珠的荧光免疫分析法定量检测鸡肉和牛奶中的磺胺甲噁唑。
Quantum Dot Nanobeads Based Fluorescence Immunoassay for the Quantitative Detection of Sulfamethazine in Chicken and Milk.
机构信息
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China.
出版信息
Sensors (Basel). 2021 Oct 3;21(19):6604. doi: 10.3390/s21196604.
Sulfamethazine (SMZ) as a broad antibiotic is widely used in livestock and poultry. However, the abuse of SMZ in livestock feed can lead to SMZ residues in food and the resistance of bacteria to drugs. Thus, a method for the detection of SMZ in food is urgently needed. In this study, quantum dot (QD) nanobeads (QBs) were synthesized by encapsulating CdSe/ZnS QDs using a microemulsion technique. The prepared QBs as signal probes were applied in lateral flow immunoassay (LFIA) for the detection of SMZ in chicken and milk. Our proposed method had limits of detection of 0.1138-0.0955 ng/mL and corresponding linear ranges of 0.2-12.5, 0.1-15 ng/mL in chicken and milk samples, respectively. The recovery of LFIA for the detection of SMZ was 80.9-109.4% and 84-101.6% in chicken and milk samples, respectively. Overall, the developed QBs-LFIA had high reliability and excellent potential for rapid and sensitive screening of SMZ in food.
磺胺甲恶唑（SMZ）作为一种广谱抗生素，广泛应用于畜牧业和家禽养殖业。然而，SMZ 在饲料中的滥用会导致其在食物中残留，并使细菌产生耐药性。因此，迫切需要一种检测食品中 SMZ 的方法。本研究采用微乳液法合成了量子点纳米珠（QBs），并用其包被 CdSe/ZnS QDs。所制备的 QBs 作为信号探针，应用于侧向流动免疫分析（LFIA），用于检测鸡肉和牛奶中的 SMZ。我们提出的方法在鸡肉和牛奶样品中的检测限分别为 0.1138-0.0955ng/mL 和 0.2-12.5、0.1-15ng/mL，相应的线性范围。LFIA 法检测鸡肉和牛奶中 SMZ 的回收率分别为 80.9-109.4%和 84-101.6%。总的来说，开发的 QBs-LFIA 具有高可靠性和优异的潜力，可用于快速灵敏地筛选食品中的 SMZ。
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