Lee Won Jeong, Moon Jae Sun, Kim Young Tae, Bach Tran The, Hai Do Van, Kim Sung Uk
Division of Systems Biology and Bioengineering, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
Vietnam Academy of Science and Technology (VAST), Hanoi 10307, Vietnam.
J Microbiol Biotechnol. 2016 Oct 28;26(10):1696-1700. doi: 10.4014/jmb.1605.05063.
In order to discover plant-derived signaling pathway inhibitors with antifungal properties, a two-component screening system utilizing the calcineurin and Hog1 mitogen-activated protein kinase pathways responsible for the virulence networks of was employed, owing to the counter-regulatory actions of these pathways. Of the 1,000 plant extracts tested, two bioactive compounds from were found to act specifically on the calcineurin pathway of . These compounds, identified as pashanone and 5-hydroxy-6,7-dimethoxyflavanone, exhibited potent antifungal activities against various human pathogenic fungi with minimum inhibitory concentration values ranging from 4.0 to >128 μg/ml.
为了发现具有抗真菌特性的植物源信号通路抑制剂，由于这些通路的反向调节作用，采用了一种利用负责毒力网络的钙调神经磷酸酶和Hog1丝裂原活化蛋白激酶通路的双组分筛选系统。在测试的1000种植物提取物中，发现两种来自[具体植物]的生物活性化合物特异性作用于[具体真菌]的钙调神经磷酸酶通路。这些化合物被鉴定为帕山酮和5-羟基-6,7-二甲氧基黄酮，对多种人类致病真菌表现出强大的抗真菌活性，最低抑菌浓度值范围为4.0至>128μg/ml。
