Department of Botany, St. Joseph College (Autonomous), Tamil Nadu, Tiruchirappalli, India, 620002.
Bioprospecting Laboratory, Department of Botany, Bharathiar University, Tamil Nadu, Coimbatore, India, 641046.
Appl Biochem Biotechnol. 2023 Nov;195(11):6790-6808. doi: 10.1007/s12010-023-04396-y. Epub 2023 Mar 17.
Miliusa nilagirica, a rare tree species of Western Ghats, belongs to the Annonaceae family, a family with potential antioxidant and antidiabetic properties. This study is designed vividly to establish the relationship between the constituent phytochemicals and their hyperglycemic effects through the antioxidant traits of M. nilagirica in vitro. Phytochemical tests were conducted on dry powdered leaves and extracts of various methods to determine the existence of various constituents. The antidiabetic potential of leaf extracts was estimated by using the α-amylase inhibitory model and the antioxidant potential was estimated with various assays. The quantitative phytochemical screening of leaf parts shows the presence of carbohydrates (88.74 ± 0.65 mg GE/g sample), proteins (82.17 ± 2.52 mg BSAE/g sample), phenolics (40.44 ± 0.43 GAE/100 g), and flavonoids (66.05 ± 0.48 mg RE/g extract). Methanol extract of Soxhlet of M. nilagirica registered the strongest antioxidant activity in all assays, 75.66% inhibition (DPPH assay), 795.01 µmol/g (ABTS˙ radical scavenging), 994.33 µmol/g (FRAP assay), 362.02 mg AAE/g extract (TAC assay), 47% inhibition (NO scavenging assay). In vitro α-amylase inhibition showed a highly noticeable reduction in ethyl acetate extract from Soxhlet (75.19%). HPLC and FTIR analyses on the extracts added strengths to the obtained results on the potentiality of M. nilagirica. From the results, it is evident that phytochemicals from M. nilagirica can be studied further, isolated, and incorporated as an alternative to synthetic supplements for hyperglycemia.
米利苏斯·尼拉吉里卡（Miliusa nilagirica）是西高止山脉的一种珍稀树种，属于番荔枝科（Annonaceae），该科具有潜在的抗氧化和抗糖尿病特性。本研究旨在通过体外研究米利苏斯·尼拉吉里卡的抗氧化特性，生动地建立其化学成分与其高血糖作用之间的关系。对干叶粉和各种方法提取的提取物进行了植物化学测试，以确定各种成分的存在。采用α-淀粉酶抑制模型估算叶提取物的抗糖尿病潜力，并用各种测定法估算抗氧化潜力。叶部分的定量植物化学筛选表明存在碳水化合物（88.74±0.65mg GE/g 样品）、蛋白质（82.17±2.52mg BSAE/g 样品）、酚类（40.44±0.43GAE/100g）和类黄酮（66.05±0.48mg RE/g 提取物）。索氏提取的甲醇提取物在所有测定中均显示出最强的抗氧化活性，抑制率为 75.66%（DPPH 测定法），795.01µmol/g（ABTS˙自由基清除），994.33µmol/g（FRAP 测定法），362.02mg AAE/g 提取物（TAC 测定法），47%的抑制（NO 清除测定法）。体外α-淀粉酶抑制实验表明，索氏提取的乙酸乙酯提取物具有显著的抑制作用（75.19%）。对提取物的 HPLC 和 FTIR 分析增强了对米利苏斯·尼拉吉里卡潜力的研究结果。结果表明，米利苏斯·尼拉吉里卡的植物化学物质可以进一步研究、分离，并作为高血糖症的合成补充物的替代品。
