Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, 94305 CA, USA.
Department of Radiology, Stanford University School of Medicine, Stanford, 94305 CA, USA.
Sci Rep. 2016 Jul 1;6:29015. doi: 10.1038/srep29015.
The sharing of circulation between two animals using a surgical procedure known as parabiosis has created a wealth of information towards our understanding of physiology, most recently in the neuroscience arena. The systemic milieu is a complex reservoir of tissues, immune cells, and circulating molecules that is surprisingly not well understood in terms of its communication across organ systems. While the model has been used to probe complex physiological questions for many years, critical parameters of recovery and exchange kinetics remain incompletely characterized, limiting the ability to design experiments and interpret results for complex questions. Here we provide evidence that mice joined by parabiosis gradually recover much physiology relevant to the study of brain function. Specifically, we describe the timecourse for a variety of recovery parameters, including those for general health and metabolism, motor coordination, activity, and sleep behavior. Finally, we describe the kinetics of chimerism for several lymphocyte populations as well as the uptake of small molecules into the brains of mice following parabiosis. Our characterization provides an important resource to those attempting to understand the complex interplay between the immune system and the brain as well as other organ systems.
通过一种名为“并体共生”的外科手术，让两只动物的循环系统共享，这为我们理解生理学创造了丰富的信息，最近在神经科学领域尤其如此。全身环境是一个复杂的组织、免疫细胞和循环分子的储存库，就其在器官系统之间的交流而言，人们对其了解甚少。虽然该模型多年来一直被用于探究复杂的生理问题，但恢复和交换动力学的关键参数仍未得到充分描述，限制了设计实验和解释复杂问题结果的能力。在这里，我们提供的证据表明，通过并体共生连接起来的老鼠逐渐恢复了许多与大脑功能研究相关的生理机能。具体来说，我们描述了各种恢复参数的时间进程，包括一般健康和代谢、运动协调、活动和睡眠行为。最后，我们描述了几种淋巴细胞群的嵌合体动力学以及小分子进入并体共生老鼠大脑的吸收情况。我们的描述为那些试图了解免疫系统和大脑以及其他器官系统之间复杂相互作用的人提供了重要的资源。
