Kim Y J, Ku S-Y, Kim Y Y, Suh C S, Kim S H, Choi Y M
Department of Obstetrics and Gynecology, Korea University Guro Hospital, Korea University College of Medicine, Seoul, South Korea.
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, South Korea.
Geburtshilfe Frauenheilkd. 2016 Jun;76(6):704-708. doi: 10.1055/s-0041-111173.
Recent animal studies demonstrated that regulating the microRNA (miRNA) in granulosa cells (GCs) modulates the meiotic competence of oocytes. However, the difference in expression profiles of miRNAs in human GCs according to the maturity of the oocyte still remains to be elucidated. This observational study investigated whether the miRNA profile of human GCs differs according to the maturity of the retrieved oocyte after controlled ovarian stimulation for in vitro fertilization (IVF). Ten women who underwent ovarian stimulation cycles with GnRH agonist long protocols were recruited. The follicular fluid (FF) from dominant follicles was individually aspirated at oocyte retrieval. Oocytes were divided into two groups according to oocyte maturity ("mature group" vs. "immature group"). GCs were collected from the FF and miRNA was analyzed using real-time PCR. Mean number of MII oocytes in the mature group was 1.6 ± 0.9 with none in the immature group (p = 0.008). Mean number of MI oocytes was 5.6 ± 2.1 in the mature group and 1.0 ± 0.0 in the immature group (p = 0.008). The total number of retrieved oocytes was 8.8 ± 1.9 in the mature group and 2.0 ± 1.2 in the immature group (p = 0.008). The GCs of the mature group showed a significantly lower expression of hsa-let-7b compared to the GCs of the immature group (p < 0.001). Taken together, the miRNA expression profiles of human GCs obtained from dominant follicles are associated with maturity of the adjacent oocyte and may be useful as a prognosticator of IVF outcome.
近期的动物研究表明，调节颗粒细胞（GCs）中的微小RNA（miRNA）可调节卵母细胞的减数分裂能力。然而，人类GCs中miRNA的表达谱根据卵母细胞成熟度的差异仍有待阐明。这项观察性研究调查了在体外受精（IVF）的控制性卵巢刺激后，人类GCs的miRNA谱是否根据回收卵母细胞的成熟度而有所不同。招募了10名接受促性腺激素释放激素（GnRH）激动剂长方案卵巢刺激周期的女性。在取卵时分别抽吸优势卵泡的卵泡液（FF）。根据卵母细胞成熟度将卵母细胞分为两组（“成熟组”与“未成熟组”）。从FF中收集GCs，并使用实时聚合酶链反应（PCR）分析miRNA。成熟组中MII期卵母细胞的平均数量为1.6±0.9，未成熟组中无（p = 0.008）。成熟组中MI期卵母细胞的平均数量为5.6±2.1，未成熟组中为1.0±0.0（p = 0.008）。成熟组回收的卵母细胞总数为8.8±1.9，未成熟组为2.0±1.2（p = 0.008）。与未成熟组的GCs相比，成熟组的GCs显示hsa-let-7b的表达显著降低（p < 0.001）。综上所述，从优势卵泡获得的人类GCs的miRNA表达谱与相邻卵母细胞的成熟度相关，并且可能作为IVF结果的预后指标。
