Chaturvedi Pratibha, Briganza Vincent
Loyola Centre for Research and Development, St. Xavier's College Campus, Ahmedabad, Gujarat, India.
Pharmacognosy Res. 2016 Jul-Sep;8(3):193-8. doi: 10.4103/0974-8490.182915.
Curculigo orchioides Gaertn (Kali musli; Family: Hypoxidaceae) is an endangered medicinal plant used for many medicinal purposes such as impotency, aphrodisiac, tonic, jaundice, and skin ailments. Its hepatoprotective, antioxidant, and anti-cancerous potential have also been evaluated by many scientists.
The objective of this study is to enhance the curculigoside content in tissue culture of C. orchioides.
The present study deals with the enhancement of an active compound of C. orchioides by incorporating various concentration of phenylalanine (Phe), tyrosine, (20, 40, 60, and 80 mg/100 ml), chromium (Cr) and nickel (Ni) (1, 2, 3, 4, and 5 ppm) into Zenk media in controlled and aseptic conditions.
Plant secondary metabolites are unique sources for pharmaceuticals, food additives, flavors, and industrially important biochemicals. Accumulation of such metabolites often occurs in plants subjected to stresses including various elicitors or signal molecules. A significantly remarkable enhancement in all induced samples was noted. Curculigoside content was maximum in the 6-week-old tissue induced with 3 ppm of Cr (7.63%) followed by 4 weeks tissue of tissue fed with 4 ppm of Ni (5.66%) and 4-week-old tissue fed with tyrosine 7.5 mg/100 ml (2.38%) among all samples used. These results suggest that tyrosine is better enhancer than Phe in the biosynthetic pathway of curculigoside. The presence of curculigoside in all extracts was confirmed by Fourier transform infrared spectroscopy, high-performance thin layer chromatography analysis with standard compound of curculigoside and histology of treated samples.
This investigation was carried out for the 1(st) time, and it is a significant step in understanding the biochemistry of curculigoside. The developed protocol will be beneficial for marketing in pharmaceutical industries.
Curculigo orchioides Gaertn (Kali musli; Family: Hypoxidaceae) is an endangered medicinal plant used for many medicinal purposes such as impotency, aphrodisiac, tonic, jaundice, and skin ailments.It was observed that dry matter % was maximum in 6-week-old tissue fed with 2.5 mg/100 ml of tyrosine and diminished beyond this concentration among all samples usedThe nickel (Ni) and chromium (Cr) stress has enhanced the curculigoside in considerable amount in nontoxic range, in tissue culture of C. orchioides.Curculigoside content was maximum in 6-week-old tissue induced with 3 ppm of Cr (7.63%; 11-fold enhancement) followed by 4 weeks tissue of tissue fed with 4 ppm of Ni (5.66%) and 4-week-old tissue fed with tyrosine 7.5 mg/100 ml (2.38%) among all samples used. Histological studies confirmed the enhanced production of curculigoside. Abbreviations Used: Phe: Phenylalanine, PAL: Phenylalanine ammonia-lyase, mM: mille Molar, Cr: Chromium, Ni: Nickel, HPTLC: High-performance thin layer chromatography.
仙茅（Curculigo orchioides Gaertn，属仙茅科，俗名卡利穆斯利）是一种濒危药用植物，可用于多种药用目的，如治疗阳痿、壮阳、滋补、黄疸和皮肤病等。许多科学家还对其肝脏保护、抗氧化和抗癌潜力进行了评估。
本研究的目的是提高仙茅组织培养中的仙茅苷含量。
本研究通过在无菌控制条件下，向岑克培养基中加入不同浓度的苯丙氨酸（Phe）、酪氨酸（20、40、60和80mg/100ml）、铬（Cr）和镍（Ni）（1、2、3、4和5ppm），来提高仙茅活性化合物的含量。
植物次生代谢产物是药物、食品添加剂、香料和具有重要工业价值的生化物质的独特来源。此类代谢产物的积累通常发生在受到包括各种诱导剂或信号分子在内的胁迫的植物中。所有诱导样品均有显著提高。在所使用的所有样品中，用3ppm Cr诱导的6周龄组织中仙茅苷含量最高（7.63%），其次是用4ppm Ni处理的4周龄组织（5.66%）和用7.5mg/100ml酪氨酸处理的4周龄组织（2.38%）。这些结果表明，在仙茅苷的生物合成途径中，酪氨酸比苯丙氨酸是更好的增强剂。通过傅里叶变换红外光谱、与仙茅苷标准化合物的高效薄层色谱分析以及处理样品的组织学检查，证实了所有提取物中仙茅苷的存在。
本研究首次开展，是了解仙茅苷生物化学的重要一步。所开发的方案将有利于在制药行业进行推广。
仙茅（Curculigo orchioides Gaertn，属仙茅科，俗名卡利穆斯利）是一种濒危药用植物，可用于多种药用目的，如治疗阳痿、壮阳、滋补、黄疸和皮肤病等。观察到在所有使用的样品中，用2.5mg/100ml酪氨酸处理的6周龄组织中干物质百分比最高，超过此浓度后干物质百分比降低。在仙茅的组织培养中，镍（Ni）和铬（Cr）胁迫在无毒范围内显著提高了仙茅苷的含量。在所使用的所有样品中，用3ppm Cr诱导的6周龄组织中仙茅苷含量最高（7.63%；提高了11倍），其次是用4ppm Ni处理的4周龄组织（5.6%）和用7.5mg/100ml酪氨酸处理的4周龄组织（2.38%）。组织学研究证实了仙茅苷产量的提高。使用的缩写：Phe：苯丙氨酸，PAL：苯丙氨酸解氨酶，mM：毫摩尔，Cr：铬，Ni：镍，HPTLC：高效薄层色谱。
