Ramchandani Dipica, Ganeshpurkar Aditya, Bansal Divya, Karchuli Manvendra Singh, Dubey Nazneen
Drug Discovery Laboratory, Shri Ram Institute of Technology-Pharmacy, Jabalpur, MP, India.
Pharmaceutics Research Laboratory, Shri Ram Institute of Technology-Pharmacy, Jabalpur, MP, India.
Toxicol Int. 2014 Sep-Dec;21(3):232-5. doi: 10.4103/0971-6580.155323.
Free radicals are one of the frequent products of normal cellular metabolism. Disparity of metabolism and excessive generation of free radicals predisposes to disorders like Parkinson's disease, Alzheimer's disease, and aging phenomenon. Curculigo orchioides Gaertn. is known for "adaptogen" and "aphrodisiac" activity and has been proved for antiasthmatic, estrogenic, antiosteoporotic activity along with protection from cisplatin-induced cell damage. C. orchioides was powdered and subjected to soxhlet extraction using methanol. Phytochemical studies and estimation of polyphenols and flavonoids was performed. Acute toxicity studies were performed by Organization for Economic Cooperation and Development OECD guidelines. Animals were treated with cyclophosphamide to induce neurotoxicity. Curculigo orchioides was powdered and subjected to soxhlet extraction using methanol. Catalase, superoxide dismutase, glutathione peroxidase, and lipid peroxidation were estimated by reported methods. C. orchioides (400 mg/kg) significantly promoted restoration of catalase (P < 0.005), superoxide dismutase (P < 0.005), and glutathion (P < 0.05) levels. Similarly, a very significant decrease (P < 0.005) in the levels of malondialdehyde was observed. In all cases as mentioned previously, C. orchioides at dose 200 mg/kg promoted significant (P < 0.05) restoration of enzyme levels. C. orchioides (Kali Musli) is rich source of phytochemicals like flavonoids and polyphenols. Flavonoids and polyphenols are reputed to demonstrate neuroprotective effect. These phytochemicals in the present study might be responsible to demonstrate neuroprotective effect.
自由基是正常细胞代谢的常见产物之一。新陈代谢的差异和自由基的过度产生易引发帕金森病、阿尔茨海默病等疾病以及衰老现象。仙茅因具有“适应原”和“壮阳”活性而闻名，并且已被证明具有抗哮喘、雌激素样、抗骨质疏松活性以及对顺铂诱导的细胞损伤的保护作用。将仙茅研磨成粉末，用甲醇进行索氏提取。进行了植物化学研究以及多酚和黄酮类化合物的测定。急性毒性研究按照经济合作与发展组织（OECD）的指导方针进行。用环磷酰胺处理动物以诱导神经毒性。将仙茅研磨成粉末，用甲醇进行索氏提取。通过报告的方法测定过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和脂质过氧化。仙茅（400毫克/千克）显著促进了过氧化氢酶（P<0.005）、超氧化物歧化酶（P<0.005）和谷胱甘肽（P<0.05）水平的恢复。同样，观察到丙二醛水平非常显著地降低（P<0.005）。在上述所有情况下，200毫克/千克剂量的仙茅促进了酶水平的显著（P<0.05）恢复。仙茅（卡利穆斯利）富含黄酮类和多酚类等植物化学物质。黄酮类和多酚类被认为具有神经保护作用。本研究中的这些植物化学物质可能是表现出神经保护作用的原因。
