Rao S R, Ravishankar G A
Laboratory of Biofunctional Materials, School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1, Asahidai, Tatsunokuchi, Ishikawa 923-1292, Japan.
Biotechnol Adv. 2002 May;20(2):101-53. doi: 10.1016/s0734-9750(02)00007-1.
This review deals with the production of high-value secondary metabolites including pharmaceuticals and food additives through plant cell cultures, shoot cultures, root cultures and transgenic roots obtained through biotechnological means. Plant cell and transgenic hairy root cultures are promising potential alternative sources for the production of high-value secondary metabolites of industrial importance. Recent developments in transgenic research have opened up the possibility of the metabolic engineering of biosynthetic pathways to produce high-value secondary metabolites. The production of the pungent food additive capsaicin, the natural colour anthocyanin and the natural flavour vanillin is described in detail.
本综述涉及通过植物细胞培养、茎尖培养、根培养以及通过生物技术手段获得的转基因根来生产包括药物和食品添加剂在内的高价值次生代谢产物。植物细胞和转基因毛状根培养是生产具有工业重要性的高价值次生代谢产物的有前景的潜在替代来源。转基因研究的最新进展为生物合成途径的代谢工程以生产高价值次生代谢产物开辟了可能性。本文详细描述了刺激性食品添加剂辣椒素、天然色素花青素和天然香料香草醛的生产。