Mohanta Sarajo, Yin Changjun, Weber Christian, Hu Desheng, Habenicht Andreas Jr
Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Munich, Germany.
Institute of Molecular Immunology, Helmholtz Zentrum München, Munich, Germany; Institute for Immunology, Ludwig-Maximilians-University, Munich, Germany.
Bio Protoc. 2016 Jun 5;6(11). doi: 10.21769/bioprotoc.1833.
Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries. Apolipoprotein E-deficient (ApoE) mice are used as experimental models to study human atherosclerosis. ApoE mice are constitutively hyperlipidemic and develop intima plaques that resemble human plaques. Various issues including experimental design for lesion analysis, dietary conditions, isolation of the aorta, staining methods, morphometry, group size, age, the location within the arterial tree, and statistical analyses are important parameters that need to be addressed to obtain robust data. Here, we provide detailed methods to quantify aorta atherosclerosis.
动脉粥样硬化是一种大中型动脉的慢性炎症性疾病。载脂蛋白E缺陷（ApoE）小鼠被用作研究人类动脉粥样硬化的实验模型。ApoE小鼠持续高脂血症，并形成类似于人类斑块的内膜斑块。包括病变分析的实验设计、饮食条件、主动脉分离、染色方法、形态测量、组大小、年龄、动脉树内的位置以及统计分析等各种问题，是获得可靠数据时需要解决的重要参数。在此，我们提供了量化主动脉粥样硬化的详细方法。
