高脂血症小鼠的主动脉粥样硬化病变分析
Aorta Atherosclerosis Lesion Analysis in Hyperlipidemic Mice.
作者信息
Mohanta Sarajo, Yin Changjun, Weber Christian, Hu Desheng, Habenicht Andreas Jr
机构信息
Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Munich, Germany.
Institute of Molecular Immunology, Helmholtz Zentrum München, Munich, Germany; Institute for Immunology, Ludwig-Maximilians-University, Munich, Germany.
出版信息
Bio Protoc. 2016 Jun 5;6(11). doi: 10.21769/bioprotoc.1833.
Abstract
Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries. Apolipoprotein E-deficient (ApoE) mice are used as experimental models to study human atherosclerosis. ApoE mice are constitutively hyperlipidemic and develop intima plaques that resemble human plaques. Various issues including experimental design for lesion analysis, dietary conditions, isolation of the aorta, staining methods, morphometry, group size, age, the location within the arterial tree, and statistical analyses are important parameters that need to be addressed to obtain robust data. Here, we provide detailed methods to quantify aorta atherosclerosis.
摘要
动脉粥样硬化是一种大中型动脉的慢性炎症性疾病。载脂蛋白E缺陷（ApoE）小鼠被用作研究人类动脉粥样硬化的实验模型。ApoE小鼠持续高脂血症，并形成类似于人类斑块的内膜斑块。包括病变分析的实验设计、饮食条件、主动脉分离、染色方法、形态测量、组大小、年龄、动脉树内的位置以及统计分析等各种问题，是获得可靠数据时需要解决的重要参数。在此，我们提供了量化主动脉粥样硬化的详细方法。
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本文引用的文献
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2
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Immunity. 2015 Jun 16;42(6):1100-15. doi: 10.1016/j.immuni.2015.05.015.
3
Artery tertiary lymphoid organs contribute to innate and adaptive immune responses in advanced mouse atherosclerosis.动脉三级淋巴器官有助于晚期动脉粥样硬化小鼠的先天和适应性免疫反应。
Circ Res. 2014 May 23;114(11):1772-87. doi: 10.1161/CIRCRESAHA.114.301137.
4
Endothelial junctional adhesion molecule-a guides monocytes into flow-dependent predilection sites of atherosclerosis.内皮细胞连接黏附分子-a 引导单核细胞进入动脉粥样硬化的依赖血流倾向部位。
Circulation. 2014 Jan 7;129(1):66-76. doi: 10.1161/CIRCULATIONAHA.113.004149. Epub 2013 Sep 24.
5
Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice.淋巴毒素β受体信号传导促进衰老的载脂蛋白E基因敲除（ApoE-/-）小鼠主动脉外膜中的三级淋巴器官生成。
J Exp Med. 2009 Jan 16;206(1):233-48. doi: 10.1084/jem.20080752. Epub 2009 Jan 12.
6
Development of experimental designs for atherosclerosis studies in mice.小鼠动脉粥样硬化研究实验设计的进展
Methods. 2005 Jun;36(2):129-38. doi: 10.1016/j.ymeth.2004.11.008.
7
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