Ullah Wazir Nadeem, Amir Khan Irfan, Javed Adil, Khan Taous, Jabbar Abdul
Cardiovascular Research Group, Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Pakistan.
Saudi J Biol Sci. 2023 Aug;30(8):103712. doi: 10.1016/j.sjbs.2023.103712. Epub 2023 Jun 16.
L () belongs to the family . A preliminary study and its medicinal use suggested its role in the management of hyperlipidemia. The present study aimed to assess the effect of methanolic root extract of in hyperlipidemia and associated vascular dysfunction. Oral administration of crude extract (Oh. Cr) to tyloxopol and high fat diet-induced hyperlipidemic Sprague-Dawley rats for 10 and 28 days significantly reduced total triglycerides and cholesterol ( < 0.001), compared to hyperlipidemic rats. Oh. Cr 250 mg/kg orally treated rats significantly ( < 0.001) reduced both the total body weight and atherogenic index in tylaxopol and HFD rats. In HMG-CoA assay, the inhibition of the enzyme was significant in Oh.Cr (250 mg/kg) treated group. Histopathological studies indicated that the group treated with Oh.Cr 250 mg/kg/day showed regular morphology of aortic intima, media and adventitia, and improved the endothelial damage. To investigate the vascular dysfunction, isolated rat aorta rings from all groups were pre-contracted with 1 µM phenylephrine (PE), and the effect of acetylcholine (Ach) was monitored. In the aorta isolated from Oh.Cr (50 mg/kg) treated group, Ach completely relaxed the PE-induced contraction with EC value of 0.05 µg/mL 0.015 (0.01-0.2) compared to the hyperlipidemic control group (<30% relaxation). In atorvastatin (10 mg/kg) treated rat aorta, Ach showed 50% relaxation. The Oh.Cr extract also reduced (105.92 ± 1.14 to 66.63 ± 0.85 mmHg) mean arterial pressure in hyperlipidemic hypertensive rats. These findings suggest that extract of is an effective remedy for hypercholesterolemia, and hypertriglyceridemia, which acts through inhibition of HMG-CoA and improving vascular dysfunction.
L（）属于该科。一项初步研究及其药用表明其在高脂血症管理中的作用。本研究旨在评估L的甲醇根提取物对高脂血症及相关血管功能障碍的影响。与高脂血症大鼠相比，给tyloxopol和高脂饮食诱导的高脂血症Sprague-Dawley大鼠口服L粗提物（Oh.Cr）10天和28天，显著降低了总甘油三酯和胆固醇（P<0.001）。Oh.Cr 250mg/kg口服治疗的大鼠显著（P<0.001）降低了tylaxopol和高脂饮食大鼠的总体重和动脉粥样硬化指数。在HMG-CoA测定中，Oh.Cr（250mg/kg）治疗组对该酶的抑制作用显著。组织病理学研究表明，Oh.Cr 250mg/kg/天治疗组的主动脉内膜、中膜和外膜形态正常，改善了内皮损伤。为了研究血管功能障碍，对所有组分离的大鼠主动脉环用1μM去氧肾上腺素（PE）进行预收缩，并监测乙酰胆碱（Ach）的作用。与高脂血症对照组（<30%舒张）相比，在Oh.Cr（50mg/kg）治疗组分离的主动脉中，Ach完全缓解了PE诱导的收缩，EC值为0.05μg/mL 0.015（0.01-0.2）。在阿托伐他汀（10mg/kg）治疗的大鼠主动脉中，Ach显示50%舒张。Oh.Cr提取物还降低了高脂血症高血压大鼠的平均动脉压（从105.92±1.14降至66.63±0.85mmHg）。这些发现表明，L提取物是治疗高胆固醇血症和高甘油三酯血症的有效药物，其作用机制是通过抑制HMG-CoA和改善血管功能障碍。
